Lin Lu-Hui, Gao Sun-Qiao, Mei Xu-Qiao, Lin Da-Yi, Chen Yi-Feng, Lin Su-Dan, Zhuang Li-Hong, Lin Cong-Meng
Department of Hematology, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou 363000, Fujian Province, China.
Department of Clinical Laboratory, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou 363000, Fujian Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2024 Aug;32(4):1085-1090. doi: 10.19746/j.cnki.issn.1009-2137.2024.04.017.
To investigate the effects of selinexor, a inhibitor of nuclear export protein 1 (XPO1) on the proliferation inhibition and apoptosis of Kasumi-1 cells in acute myeloid leukemia (AML).
MTS method was used to detect the inhibitory effect of different concentrations of selinexor on the proliferation of Kasumi-1 cells at different time points. The apoptosis rate and cell cycle changes after treatment with different concentration of selinexor were detected by flow cytometry.
Selinexor inhibited the growth of Kasumi-1 cells at different time points in a concentration-dependent manner ( =0.7592, =0.9456, and =0.9425). Selinexor inhibited Kasumi-1 cells growth in a time-dependent manner ( =0.9057 in 2.5 μmol/L group, =0.9897 in 5 μmol/L group and =0.9994 in 10 μmol/L group). Selinexor could induce apoptosis of Kasumi-1 cells in a dose-dependent manner ( =0.9732), and the apoptosis of Kasumi-1 cells was more obvious with the increase of drug concentration. The proportion of G/G phase was significantly increased and the proportion of S phase was significantly decreased after the treatment of Kasumi-1 cells by selinexor. With the increase of drug concentration, the proportion of Kasumi-1 cells cycle arrest in G/G phase was increased and the cell synthesis was decreased.
Selinexor can promote the death of tumor cells by inhibiting Kasumi-1 cells proliferation, inducing apoptosis and blocking cell cycle.
探讨核输出蛋白1（XPO1）抑制剂塞利尼索对急性髓系白血病（AML）Kasumi-1细胞增殖抑制及凋亡的影响。
采用MTS法检测不同浓度塞利尼索在不同时间点对Kasumi-1细胞增殖的抑制作用。采用流式细胞术检测不同浓度塞利尼索处理后细胞凋亡率及细胞周期变化。
塞利尼索在不同时间点以浓度依赖性方式抑制Kasumi-1细胞生长（=0.7592，=0.9456，=0.9425）。塞利尼索以时间依赖性方式抑制Kasumi-1细胞生长（2.5 μmol/L组=0.9057，5 μmol/L组=0.9897，10 μmol/L组=0.9994）。塞利尼索可呈剂量依赖性诱导Kasumi-1细胞凋亡（=0.9732），且随着药物浓度增加，Kasumi-1细胞凋亡更明显。塞利尼索处理Kasumi-1细胞后，G/G期比例显著增加，S期比例显著降低。随着药物浓度增加，Kasumi-1细胞周期阻滞于G/G期的比例增加，细胞合成减少。
塞利尼索可通过抑制Kasumi-1细胞增殖、诱导凋亡及阻滞细胞周期促进肿瘤细胞死亡。
