Poll E H, Arwert F
Mutat Res. 1985 Jan-Mar;145(1-2):77-80. doi: 10.1016/0167-8817(85)90043-4.
Confluent cultures of normal human fibroblasts were treated with the chemotherapeutic agents, mitomycin C (MMC) and cis-diamminedichloroplatinum(II) (cis-Pt(II]. This treatment induced a decrease in clonogenic cell survival. Recovery of the cytotoxic effect was observed in the case of cis-Pt(II)-treated cultures maintained at confluence for 1-5 days. No such recovery was observed after treatment with MMC. These data suggest that contrary to potential lethal damage induced by cis-Pt(II) which is repaired in confluent cells, DNA damage induced by MMC is not repaired in confluent cells.
将正常人成纤维细胞的融合培养物用化疗药物丝裂霉素C（MMC）和顺二氨二氯铂（II）（顺铂（II））处理。这种处理导致克隆形成细胞存活率降低。在汇合状态下维持1至5天的顺铂（II）处理的培养物中观察到细胞毒性作用的恢复。用MMC处理后未观察到这种恢复。这些数据表明，与顺铂（II）诱导的潜在致死性损伤在汇合细胞中可修复相反，MMC诱导的DNA损伤在汇合细胞中不可修复。
