Prost Jean-Christophe, Brunner Felix, Bovet Cédric, Grob Christian, Berchtold Christian, Schlotterbeck Götz, Kröll Dino, Largiadèr Carlo R, Fiedler Georg Martin, Juillerat Pascal
University Institute of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, Switzerland.
Gastroenterology, Clinic for Visceral Surgery and Medicine, Bern University Hospital, Bern, Switzerland.
Clin Mass Spectrom. 2017 Mar 1;3:1-6. doi: 10.1016/j.clinms.2017.02.001. eCollection 2017 Jan.
Bile acids malabsorption (BAM) is encountered in numerous gastrointestinal pathologies and is a good example of a treatable cause of watery diarrhea after ileal resection. The gold standard for diagnosing BAM is the selenium homocholic acid taurine test (SeHCAT), an expensive and complex analysis. An alternative method is the quantification of 7α-hydroxy-4-cholesten-3-one (C4). Here, we present a simple, ultra high-performance liquid chromatography-tandem mass spectrometry method to measure C4 in human serum. To avoid time consuming sample preparation (e.g., derivatization, solid phase extraction), we used absorption chemistry-based extraction plates. This method demonstrates a lower limit of quantification of 5 ng/mL and is linear over a concentration range from 5 to 300 ng/mL (R = 0.9977). Inaccuracy and imprecision were less than 15%. The validated method is currently used for routine measurement of C4 from serum in patients to confirm BAM diagnosis.
胆汁酸吸收不良（BAM）在多种胃肠道疾病中都有出现，是回肠切除术后导致水样腹泻的可治疗病因的一个典型例子。诊断BAM的金标准是硒同型胆酸牛磺酸试验（SeHCAT），这是一项昂贵且复杂的分析。一种替代方法是对7α-羟基-4-胆甾烯-3-酮（C4）进行定量。在此，我们介绍一种简单的超高效液相色谱-串联质谱法来测定人血清中的C4。为避免耗时的样品制备（如衍生化、固相萃取），我们使用了基于吸附化学的萃取板。该方法的定量下限为5 ng/mL，在5至300 ng/mL的浓度范围内呈线性（R = 0.9977）。不准确度和不精密度小于15%。该经过验证的方法目前用于对患者血清中的C4进行常规测量，以确诊BAM。
