Dahlin Jayme L, Petrides Athena K
Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA.
Harvard Medical School, Boston, MA, USA.
Clin Mass Spectrom. 2018 Jul 26;9:23-24. doi: 10.1016/j.clinms.2018.07.002. eCollection 2018 Aug.
Drug monitoring of benzodiazepines is often performed in toxicology and pain management settings. During the validation of a laboratory-developed multiplex UPLC-MS/MS assay for quantifying benzodiazepines in human urine, we observed notable instability (greater than 20% nominal) in 7-aminoclonazepam (7AC) during prolonged freezing conditions by performing method comparisons and a conventional analyte stability study. These findings highlight the importance of proper calibrator storage and also the essential need to perform method comparison and analyte stability studies. It is recommended that clinical laboratories testing for urine 7AC carefully validate its stability if subjected to freezing conditions.
苯二氮䓬类药物的药物监测通常在毒理学和疼痛管理环境中进行。在验证一种实验室开发的用于定量人尿中苯二氮䓬类药物的多重超高效液相色谱-串联质谱（UPLC-MS/MS）测定法时，我们通过进行方法比较和常规分析物稳定性研究，观察到在长时间冷冻条件下7-氨基氯硝西泮（7AC）存在显著的不稳定性（大于标称值的20%）。这些发现突出了校准物妥善储存的重要性，以及进行方法比较和分析物稳定性研究的必要性。建议检测尿中7AC的临床实验室如果样品要经受冷冻条件，需仔细验证其稳定性。
