TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, 410208, Changsha, China.
Science & Technology Innovation Center, Hunan University of Chinese Medicine, 410208, Changsha, China.
Anal Methods. 2024 Sep 19;16(36):6220-6228. doi: 10.1039/d4ay01353k.
In contrast to the unipedal DNA walker, a bipedal DNA walker features a broader walking area and exhibits faster walking kinetics, leading to enhanced amplification efficiency. In this study, we designed a stochastic three-dimensional (3D) bipedal DNA walker, capable of navigating AuNP-based 3D tracks, driven by exonuclease III (Exo III). This detection system enables the linear detection of the non-invasive biomarker apurinic/apyrimidinic endonuclease 1 (APE1) activity across a range of 0 to 120 U per mL, with a detection limit of 0.03 U per mL. The platform not only offers a novel DNA walker for sensitive APE1 detection in cell lysate but also facilitates the precise assessment of NCA's inhibitory effect on APE1. This research holds promise for future screening of other potential APE1 inhibitors.
与单足 DNA walker 相比，双足 DNA walker 的行走区域更宽，表现出更快的行走动力学，从而提高了扩增效率。在这项研究中，我们设计了一种随机的三维（3D）双足 DNA walker，它能够在金纳米颗粒（AuNP）的 3D 轨道上导航，由外切酶 III（Exo III）驱动。这个检测系统可以线性检测非侵入性生物标志物脱嘌呤/脱嘧啶内切酶 1（APE1）的活性，范围为 0 到 120 U/mL，检测限为 0.03 U/mL。该平台不仅为细胞裂解液中灵敏的 APE1 检测提供了一种新型的 DNA walker，还便于精确评估 NCA 对 APE1 的抑制作用。这项研究为未来筛选其他潜在的 APE1 抑制剂提供了可能。
