编码核糖体成熟结构域蛋白的水稻基因对叶绿体发育至关重要。

The Rice Gene Encoding a Ribosome Maturation Domain Protein Is Essential for Chloroplast Development.

作者信息

Sun Yunguang, Liu Yanxia, Zhang Youze, Lin Dongzhi, Pan Xiaobiao, Dong Yanjun

机构信息

College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.

Shanghai Key Laboratory of Plant Molecular Sciences, Shanghai 200234, China.

出版信息

Biology (Basel). 2024 Jul 31;13(8):580. doi: 10.3390/biology13080580.

DOI:10.3390/biology13080580

PMID:39194518

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11351240/

Abstract

Chloroplast RNA splicing and ribosome maturation (CRM) domain proteins are a family of plant-specific proteins associated with RNA binding. In this study, we have conducted a detailed characterization of a novel rice CRM gene () mutant, , which showed yellow-green leaves at all the stages, had fewer tillers, and had a decreased plant height. Map-based cloning and CRISPR/Cas9 editing techniques all showed that encoded a CRM domain protein in rice. In addition, subcellular localization revealed that YL4 was in chloroplasts. transcripts were highly expressed in all leaves and undetectable in roots and stems, and the mutation of affected the transcription of chloroplast-development-related genes. This study indicated that is essential for chloroplast development and affects some agronomic traits.

摘要

叶绿体RNA剪接与核糖体成熟（CRM）结构域蛋白是一类与RNA结合相关的植物特异性蛋白。在本研究中，我们对一个新的水稻CRM基因（）突变体进行了详细表征，该突变体在所有阶段均表现出黄绿色叶片，分蘖较少，株高降低。图位克隆和CRISPR/Cas9编辑技术均表明，在水稻中编码一种CRM结构域蛋白。此外，亚细胞定位显示YL4存在于叶绿体中。转录本在所有叶片中高度表达，在根和茎中未检测到，并且的突变影响了叶绿体发育相关基因的转录。本研究表明，对叶绿体发育至关重要，并影响一些农艺性状。