Thompson L H, Mooney C L, Burkhart-Schultz K, Carrano A V, Siciliano M J
Somat Cell Mol Genet. 1985 Jan;11(1):87-92. doi: 10.1007/BF01534738.
A UV-sensitive mutant line of CHO cells, UV20, was shown to be phenotypically corrected to resistance by fusion with human lymphocytes or fibroblasts. Only human chromosome 19 correlated with the DNA repair phenotype of resistant hybrid clones and their resistant or sensitive subclones. This study demonstrates the mapping of a human repair gene by direct selection of complementing hybrids in the presence of a DNA-damaging agent (mitomycin C).
中国仓鼠卵巢细胞（CHO）的一个紫外线敏感突变株UV20，经与人淋巴细胞或成纤维细胞融合后，在表型上被校正为具有抗性。只有人类19号染色体与抗性杂交克隆及其抗性或敏感亚克隆的DNA修复表型相关。本研究证明了在存在DNA损伤剂（丝裂霉素C）的情况下，通过直接选择互补杂种来定位人类修复基因。
