Wu Haili, Yuan Haiyan, Duan Yiwei, Li Guangjun, Du Jin'e, Wang Panfeng, Li Zhuoyu
College of Life Science, Shanxi University, Taiyuan 030006, China.
Institute of Biotechnology, The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China.
Biology (Basel). 2024 Aug 22;13(8):644. doi: 10.3390/biology13080644.
Hepatocellular carcinoma (HCC) is one of the malignant tumors with high morbidity and mortality. Long non-coding RNAs (lncRNAs) are frequently dysregulated in human cancers and play an important role in the initiation and progression of HCC. Here, we investigated the expression of a new reported lncRNA495810 in our previous study by analyzing the publicly available datasets and using RT-qPCR assay. The cell proliferation experiment, cell cycle and apoptosis assay, wound healing assay, cell migration assay were used to explore the biological function of lncRNA495810 in HCC. The western blot, RNA pull down and RNA immunoprecipitation (RIP) detection were used to investigate the potential molecular mechanisms of lncRNA495810. The results demonstrated that lncRNA495810 was significantly upregulated in hepatocellular carcinoma and associated with poor prognosis of hepatocellular carcinoma patients. Moreover, it proved that lncRNA495810 promotes the proliferation and metastasis of hepatoma cells by directly binding and upregulating the expression of fatty acid-binding protein 5. These results reveal the oncogenic roles of lncRNA495810 in HCC and provide a potential therapeutic target for HCC.
肝细胞癌（HCC）是发病率和死亡率较高的恶性肿瘤之一。长链非编码RNA（lncRNAs）在人类癌症中经常失调，并在HCC的发生和发展中起重要作用。在此，我们通过分析公开可用数据集并使用RT-qPCR检测法，研究了我们之前研究中一种新报道的lncRNA495810的表达情况。采用细胞增殖实验、细胞周期和凋亡检测、伤口愈合检测、细胞迁移检测来探究lncRNA495810在HCC中的生物学功能。使用蛋白质免疫印迹、RNA下拉和RNA免疫沉淀（RIP）检测来研究lncRNA495810的潜在分子机制。结果表明，lncRNA495810在肝细胞癌中显著上调，且与肝细胞癌患者的不良预后相关。此外，证明lncRNA495810通过直接结合并上调脂肪酸结合蛋白5的表达来促进肝癌细胞的增殖和转移。这些结果揭示了lncRNA495810在HCC中的致癌作用，并为HCC提供了一个潜在的治疗靶点。
