Barough Robab Ebrahimi, Javidnia Javad, Davoodi Ali, Talebpour Amiri Fereshteh, Moazeni Maryam, Sarvi Shahabeddin, Valadan Reza, Siahposht-Khachaki Ali, Moosazadeh Mahmood, Nosratabadi Mohsen, Haghani Iman, Meis Jacques F, Abastabar Mahdi, Badali Hamid
Student Research Committee, Mazandaran University of Medical Sciences, Sari 48157-33971, Iran.
Invasive Fungi Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari 48157-33971, Iran.
J Fungi (Basel). 2024 Jul 25;10(8):518. doi: 10.3390/jof10080518.
, an emerging non- multidrug-resistant yeast, has become a significant cause of invasive candidiasis in healthcare settings. So far, data on the metabolites of in different clades are minimal, and no studies have focused on clade V metabolites. Therefore, Gas chromatography-mass spectrometry (GC-MS) was used for the metabolomic profiling of clade I compared with fluconazole-resistant and susceptible in clade V strains. GC-MS chromatography revealed 28, 22, and 30 compounds in methanolic extracts of the fluconazole-susceptible and fluconazole-resistant clade V and clade I strain, respectively. Some compounds, such as acetamide and metaraminol, were found in fluconazole-susceptible and resistant clade V and clade I. N-methyl-ethanamine and bis(2-ethylhexyl) phthalate metabolites were found in both fluconazole -susceptible and resistant clade V, as well as 3-methyl-4-isopropylphenol, 3,5-bis(1,1-dimethyl)-1,2-benzenediol, and diisostyl phthalate metabolites in both fluconazole resistant clade V and I. Identifying these metabolites contributes to understanding the morphogenesis and pathogenesis of , highlighting their potential role in antifungal drug resistance and the control of fungal growth. However, further experiments are warranted to fully comprehend the identified metabolites' regulatory responses, and there may be potential challenges in translating these findings into clinical applications.
一种新兴的非多重耐药酵母已成为医疗机构侵袭性念珠菌病的重要病因。到目前为止，关于不同进化枝中该酵母代谢物的数据极少，且尚无研究聚焦于进化枝V的代谢物。因此，采用气相色谱 - 质谱联用（GC - MS）对进化枝I的该酵母进行代谢组学分析，并与进化枝V菌株中对氟康唑耐药和敏感的该酵母进行比较。GC - MS色谱分析分别在对氟康唑敏感和耐药的进化枝V及进化枝I的该酵母甲醇提取物中检测到28种、22种和30种化合物。在对氟康唑敏感和耐药的进化枝V及进化枝I中均发现了一些化合物，如乙酰胺和间羟胺。在对氟康唑敏感和耐药的进化枝V中均发现了N - 甲基乙胺和邻苯二甲酸二（2 - 乙基己基）酯代谢物，在对氟康唑耐药的进化枝V和I中均发现了3 - 甲基 - 4 - 异丙基苯酚、3,5 - 双（1,1 - 二甲基）- 1,2 - 苯二酚和邻苯二甲酸二异司替酯代谢物。鉴定这些代谢物有助于理解该酵母的形态发生和发病机制，突出它们在抗真菌耐药性及真菌生长控制中的潜在作用。然而，有必要进行进一步实验以全面理解所鉴定代谢物的调节反应，并且将这些发现转化为临床应用可能存在潜在挑战。
