Faculty of Medicine, Autonomous University of Sinaloa, Culiacán 80246, Mexico.
Programa de Maestría en Ciencias en Biomedicina Molecular, Facultad de Medicina, Universidad Autónoma de Sinaloa (UAS), Culiacán 80246, Mexico.
Mar Drugs. 2024 Aug 14;22(8):369. doi: 10.3390/md22080369.
Dengue, caused by the dengue virus (DENV), is a global health threat transmitted by mosquitoes, resulting in 400 million cases annually. The disease ranges from mild to severe, with potential progression to hemorrhagic dengue. Current research is focused on natural antivirals due to challenges in vector control. This study evaluates the antiviral potential of peptides derived from the microalgae , known for its bioactive compounds. Microalgae were cultivated under controlled conditions, followed by protein extraction and hydrolysis to produce four peptide fractions. These fractions were assessed for cytotoxicity via the MTT assay and antiviral activity against DENV serotype 2 using flow cytometry and plaque formation assays. The 10-30 kDa peptide fraction, at 150 and 300 μg/mL concentrations, demonstrated no cytotoxicity and significantly reduced the percentage of infected cells and viral titers. These findings suggest that peptides derived from exhibit promising antiviral activity against dengue virus serotype 2, potentially contributing to developing new therapeutic approaches for dengue.
登革热是由登革病毒（DENV）引起的一种全球性健康威胁，通过蚊子传播，每年导致 4 亿例病例。该疾病的严重程度从轻度到重度不等，有发展为出血性登革热的可能。由于在控制病媒方面存在挑战，目前的研究集中在天然抗病毒药物上。本研究评估了源自微藻的肽的抗病毒潜力，微藻因其生物活性化合物而闻名。在控制条件下培养微藻，然后进行蛋白质提取和水解，产生四个肽级分。通过 MTT 测定法评估这些级分的细胞毒性，并通过流式细胞术和蚀斑形成测定法评估它们对 DENV 血清型 2 的抗病毒活性。10-30 kDa 肽级分在 150 和 300 μg/mL 浓度下无细胞毒性，并显著降低感染细胞的百分比和病毒滴度。这些发现表明，源自 的肽对登革热病毒血清型 2具有有前途的抗病毒活性，可能有助于开发新的登革热治疗方法。
