Ismail Hammad, Mubashar Zaryab, Khan Hajra, Naveed Zeenat, Dilshad Erum, Bhatti Muhammad Zeeshan, Anwaar Sadaf, Saleem Samreen, Mehmood Sabba, Rahman Abdur, Rashid Umer, Fouad Dalia, De Waard Michel, Batiha Gaber El-Saber
Department of Biochemistry and Biotechnology, University of Gujrat, Gujrat 50700, Pakistan.
Department of Bioinformatics and Biosciences, Faculty of Health and Life Sciences, Capital University of Science and Technology, Islamabad 44000, Pakistan.
Metabolites. 2024 Aug 8;14(8):442. doi: 10.3390/metabo14080442.
Trans fatty acids are specific unsaturated fats found in processed foods that undergo hydrogenation, leading to hepatic disorders such as metabolic-associated fatty liver disease (MAFLD) and conditions like CVD and CKD. The effects of different food samples containing trans fatty acids (elaidic and oleic acid) on the liver, heart, and kidney through antioxidant enzyme activity were investigated in animal models. Liver function tests (ALT, ALP, AST, and LDH), heart biomarker levels (CPK, TC, HDL, LDL, and triglycerides), and kidney biomarker levels (serum creatinine, blood urea nitrogen, and serum uric acid) were examined in serum of rabbits and the histopathology of liver tissues. Results showed that these biomarkers were more elevated in the Mujahid Ghee group than in the normal control, oleic acid, and Kausar Ghee groups. The concentration of antioxidant markers such as peroxidase, glutathione, catalase, thiobarbituric acid reactive substances, and superoxide dismutase were lower in the Mujahid Ghee group. HPLC showed that Mujahid Ghee had the highest quantified value of elaidic acid among all selected samples. Overall, this study demonstrated that elaidic acid in its purest form aggravated MAFLD in rabbit livers and provoked CVK and CVD.
反式脂肪酸是在经过氢化处理的加工食品中发现的特定不饱和脂肪，会导致肝脏疾病，如代谢相关脂肪性肝病（MAFLD）以及心血管疾病（CVD）和慢性肾脏病（CKD）等病症。在动物模型中研究了含有反式脂肪酸（反油酸和油酸）的不同食物样本通过抗氧化酶活性对肝脏、心脏和肾脏的影响。检测了兔血清中的肝功能指标（谷丙转氨酶、碱性磷酸酶、谷草转氨酶和乳酸脱氢酶）、心脏生物标志物水平（肌酸磷酸激酶、总胆固醇、高密度脂蛋白、低密度脂蛋白和甘油三酯）以及肾脏生物标志物水平（血清肌酐、血尿素氮和血清尿酸），并对肝脏组织进行了组织病理学检查。结果显示，与正常对照组、油酸组和考萨尔酥油组相比，穆贾希德酥油组的这些生物标志物升高更为明显。穆贾希德酥油组中过氧化物酶、谷胱甘肽、过氧化氢酶、硫代巴比妥酸反应性物质和超氧化物歧化酶等抗氧化标志物的浓度较低。高效液相色谱法显示，在所有选定样本中，穆贾希德酥油的反油酸定量值最高。总体而言，本研究表明，最纯净形式的反油酸会加重兔肝脏中的MAFLD，并引发CVK和CVD。
