Cerda-Apresa Daniela, Gutierrez-Rodriguez Selene M, Davila-Barboza Jesus A, Lopez-Monroy Beatriz, Rodriguez-Sanchez Iram P, Saavedra-Rodriguez Karla L, Flores Adriana E
Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, Av. Universidad s/n Cd. Universitaria, San Nicolas de los Garza 66455, NL, Mexico.
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
Trop Med Infect Dis. 2024 Aug 18;9(8):184. doi: 10.3390/tropicalmed9080184.
The growing resistance of (L.) to conventional insecticides presents a major challenge in arbovirus control, necessitating the exploration of alternative insecticidal chemistries. Spiromesifen, derived from spirocyclic tetronic acids, is widely used against agricultural pests and is crucial in resistance management due to its unique lipid synthesis inhibition. This study evaluates the insecticidal activity of spiromesifen against temephos-resistant populations, focusing on larval body weight, volume, biochemical composition, and adult female reproductive potential. Spiromesifen demonstrated effective larvicidal activity, significantly reducing adult emergence. Resistance to spiromesifen was not observed, with resistance ratios (RR, RR) ranging from 0.36- to 3.31-fold. Larvae exposed to LC showed significant reductions in body weight and volume, and reduced carbohydrate, lipid, and protein contents. Enhanced catalase activity and malondialdehyde levels indicated increased oxidative stress and lipid peroxidation, highlighting its effects on lipid metabolism. Spiromesifen also exhibited sterilizing effects, significantly reducing fecundity and fertility in adult females, thereby impacting reproductive capacity. These findings highlight the potential of spiromesifen as a component of integrated vector management strategies, especially in regions with prevalent insecticide resistance in , serving as an effective larvicide and impacting adult reproductive outcomes.
（L.）对传统杀虫剂的抗性不断增强，这给虫媒病毒控制带来了重大挑战，因此有必要探索替代杀虫化学物质。螺虫乙酯源自螺环特窗酸，广泛用于防治农业害虫，因其独特的脂质合成抑制作用，在抗性管理中至关重要。本研究评估了螺虫乙酯对耐倍硫磷（L.）种群的杀虫活性，重点关注幼虫体重、体积、生化组成以及成年雌虫的生殖潜力。螺虫乙酯表现出有效的杀幼虫活性，显著降低了成虫羽化率。未观察到对螺虫乙酯的抗性，抗性比率（RR，RR）在0.36至3.31倍之间。暴露于LC的幼虫体重和体积显著降低，碳水化合物、脂质和蛋白质含量减少。过氧化氢酶活性增强和丙二醛水平升高表明氧化应激和脂质过氧化增加，突出了其对脂质代谢的影响。螺虫乙酯还表现出绝育作用，显著降低成年雌虫的繁殖力和生育力，从而影响（L.）的生殖能力。这些发现突出了螺虫乙酯作为综合病媒管理策略组成部分的潜力，特别是在（L.）对杀虫剂抗性普遍存在的地区，可作为一种有效的杀幼虫剂并影响成虫繁殖结果。
