Centro Regional de Investigación en Salud Pública, Instituto Nacional de Salud Pública/Departamento de Agricultura, Sociedad y Ambiente, Ecología de Artrópodos y Manejo de Plagas, El Colegio de la Frontera Sur. Tapachula, Chiapas, Mexico.
Centro Regional de Investigación en Salud Pública, Instituto Nacional de Salud Pública. Tapachula, Chiapas, Mexico.
Salud Publica Mex. 2023 Jan 2;65(1, ene-feb):19-27. doi: 10.21149/13910.
To identify the enzyme-mediated insecticide resistance in Aedes aegypti in Tapachula, Mexico.
Biochemical assays were undertaken to determine the enzyme levels in mosquitoes from 22 sites collected in 2018 and 2020 in Tapachula. Results of 2018 were correlated with the resistance to insecticides pub-lished.
Mosquitoes had higher levels than those of the susceptible strain in 2018 and 2020 respectively of α-esterases in 15 and 12 sites; β-esterases in 7 and 6 sites; glutathione-S-transferases in 11 and 19 sites; ρNPA-esterases in 21 and 17 sites; and cytochromes P450 in 20 and 22 sites. In mosquitoes of 2018, there was a moderate correlation between previously documented Malathion resistance ratios and the insensitive acetylcholinesterase (r=0.459, p= 0.03).
The elevated enzyme levels found indicate its contribution to the resistance to pyrethroids and organo-phosphates already published in mosquitoes from Tapachula. Bioassays using enzyme inhibitors resulted in greater mor-tality, confirming that metabolism contributes to resistance.
鉴定墨西哥塔帕楚拉伊蚊体内介导的杀虫剂抗性相关酶。
2018 年和 2020 年，在塔帕楚拉的 22 个地点采集了蚊子，并进行了生化测定以确定其体内的酶水平。将 2018 年的结果与已发表的抗药性进行了相关性分析。
与敏感株相比，2018 年和 2020 年，15 个和 12 个地点的蚊子体内α-酯酶水平分别升高；7 个和 6 个地点的β-酯酶水平升高；11 个和 19 个地点的谷胱甘肽 S-转移酶水平升高；21 个和 17 个地点的 ρNPA-酯酶水平升高；20 个和 22 个地点的细胞色素 P450 水平升高。在 2018 年的蚊子中，先前记录的马拉硫磷抗性比与不敏感乙酰胆碱酯酶之间存在中度相关性（r=0.459，p=0.03）。
已发现的酶水平升高表明其对塔帕楚拉伊蚊中已发表的拟除虫菊酯和有机磷杀虫剂的抗性具有一定贡献。使用酶抑制剂的生物测定导致更高的死亡率，证实了代谢对抗性的贡献。
