Song Mingxuan, Ruan Qinli, Wang Dayong
School of Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Medical School, Southeast University, Nanjing 210009, China.
Toxics. 2024 Jul 30;12(8):555. doi: 10.3390/toxics12080555.
Increasing evidence has suggested that nanoplastic pollution has become a global concern. More importantly, transgenerational toxicity can be induced by nanoplastics at predicted environmentally relevant doses (ERDs). Considering that amino modification could increase nanoplastic toxicity, we compared transgenerational neurotoxicity between pristine polystyrene nanoparticle (PS-NP) and amino-modified PS-NP (NH-PS-NP) in . At 0.1-10 μg/L, NH-PS-NP caused more severe transgenerational toxicity on locomotion and neuronal development. Accompanied with a difference in transgenerational neuronal damage, compared to PS-NP (10 μg/L), NH-PS-NP (10 μg/L) induced more severe transgenerational activation of , , , and , which are required for the induction of neurodegeneration. Moreover, NH-PS-NP (10 μg/L) caused more severe transgenerational inhibition in expressions of , , , and than PS-NP (10 μg/L), and RNA interference (RNAi) of these genes conferred susceptibility to the toxicity of PS-NP and NH-PS-NP on locomotion and neuronal development. NH-PS-NP (10 μg/L) further caused more severe transgenerational activation of germline ligand genes (, , , , , , and ) than PS-NP (10 μg/L), and RNAi of these ligand genes caused resistance to the toxicity of PS-NP and NH-PS-NP on locomotion and neuronal development. Our results highlighted more severe exposure risk of amino-modified nanoplastics at ERDs in causing transgenerational neurotoxicity in organisms.
越来越多的证据表明，纳米塑料污染已成为全球关注的问题。更重要的是，纳米塑料在预测的环境相关剂量（ERDs）下可诱导跨代毒性。考虑到氨基修饰会增加纳米塑料的毒性，我们比较了原始聚苯乙烯纳米颗粒（PS-NP）和氨基修饰的PS-NP（NH-PS-NP）之间的跨代神经毒性。在0.1-10μg/L时，NH-PS-NP对运动和神经元发育造成更严重的跨代毒性。与跨代神经元损伤的差异相伴，与PS-NP（10μg/L）相比，NH-PS-NP（10μg/L）诱导了更多、、、和的跨代激活，这些是诱导神经退行性变所必需的。此外，NH-PS-NP（10μg/L）比PS-NP（10μg/L）对、、、和的表达造成更严重的跨代抑制，并且这些基因的RNA干扰（RNAi）使对PS-NP和NH-PS-NP在运动和神经元发育方面的毒性敏感。NH-PS-NP（10μg/L）比PS-NP（10μg/L）进一步导致种系配体基因（、、、、、、和）更严重的跨代激活，并且这些配体基因的RNAi导致对PS-NP和NH-PS-NP在运动和神经元发育方面的毒性产生抗性。我们的结果突出了在环境相关剂量下氨基修饰的纳米塑料在导致生物体跨代神经毒性方面更严重的暴露风险。
