Department of Emergency Medicine, Shandong Provincial Third Hospital, Shandong University, 250031, Jinan, China.
Department of Respiratory, Qingdao, Qingdao Central Hospital, University of Health and Rehabilitation Sciences (Qingdao Central Hospital), 266042, Qingdao, China.
Dokl Biochem Biophys. 2024 Oct;518(1):442-451. doi: 10.1134/S160767292460043X. Epub 2024 Aug 28.
Acute kidney injury (AKI), formerly known as acute renal failure, refers to a sudden and often reversible decline in kidney function. Inflammatory reaction and oxidative stress play a crucial role in the expansion of renal disease. In this experimental study, we scrutinized the renal protective effect of umbelliferone against gentamicin induced renal injury in the rats and explore the mechanism. Wistar rats were used in this study and Gentamicin was used for the induction the AKI in the rats and rats were received the oral administration of umbelliferone. The body weight, organ weight, renal, oxidative stress, cytokines, inflammatory parameters were estimated. The mRNA expression caspase-3, Bax, Bcl-2, TNF-α, IL-1β, IL-6, IL-10, HO-1, and Nrf2 were estimated. Umbelliferone remarkably improved the body weight and altered the absolute and relative weight of hepatic and renal tissue. Umbelliferone significantly suppressed the level of BUN, Scr, magnesium, calcium, phosphorus, sodium, and potassium along with altered the level of oxidative stress parameters like CAT, SOD, GSH, LPO, and GPx. Umbelliferone altered the level of cytokines viz., TNF-α, Il-1β, IL-6, IL-10; inflammatory parameters like PGE2, COX-2, TGF-β, NF-κB, respectively. Umbelliferone significantly altered the mRNA expression of caspase-3, Bax, Bcl-2, TNF-α, IL-1β, IL-6, IL-10, HO-1, and Nrf2. The result showed the renal protective effect of umbelliferone against gentamycin induced renal disease via alteration of HO-1/Nrf2 and NF-κB Signaling Pathway.
急性肾损伤（AKI），以前称为急性肾衰竭，是指肾功能的突然而经常是可逆的下降。炎症反应和氧化应激在肾脏疾病的扩展中起着关键作用。在这项实验研究中，我们仔细研究了伞形酮对庆大霉素诱导的大鼠肾损伤的肾保护作用，并探讨了其机制。本研究使用 Wistar 大鼠，用庆大霉素诱导 AKI，大鼠口服伞形酮。估计体重、器官重量、肾、氧化应激、细胞因子、炎症参数。估计 caspase-3、Bax、Bcl-2、TNF-α、IL-1β、IL-6、IL-10、HO-1 和 Nrf2 的 mRNA 表达。伞形酮显著改善了体重，并改变了肝和肾组织的绝对和相对重量。伞形酮显著抑制 BUN、Scr、镁、钙、磷、钠和钾的水平，并改变 CAT、SOD、GSH、LPO 和 GPx 等氧化应激参数的水平。伞形酮改变了细胞因子 TNF-α、IL-1β、IL-6、IL-10 的水平；炎症参数如 PGE2、COX-2、TGF-β、NF-κB。伞形酮显著改变了 caspase-3、Bax、Bcl-2、TNF-α、IL-1β、IL-6、IL-10、HO-1 和 Nrf2 的 mRNA 表达。结果表明，伞形酮通过改变 HO-1/Nrf2 和 NF-κB 信号通路对庆大霉素诱导的肾病具有肾保护作用。
