缺氧诱导因子-1 通路介导红景天苷通过自分泌方式对破骨细胞分化的作用
The autocrine action of salidroside on osteoclast during osteoclastogenesis via hypoxia-inducible factor-1 pathway.
机构信息
School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Department of Orthodontic, Tianjin Stomatological Hospital, Tianjin, China.
出版信息
Hum Exp Toxicol. 2024 Jan-Dec;43:9603271241269028. doi: 10.1177/09603271241269028.
BACKGROUND AND OBJECTIVE
The objective of this study was to investigate the potential of salidroside (SAL) (a major active compound in L.) in regulating osteoclast differentiation and function by modulating the HIF-1 pathway and its downstream target genes.
METHODS
The expression of HIF-1 and its downstream target genes was examined at both mRNA and protein levels in osteoclasts treated with SAL. Immunofluorescence analysis was performed to assess the nuclear translocation and transcriptional activity of HIF-1 in response to SAL. MTT, flow cytometry, qPCR, TRAP staining and bone resorption assays were used to evaluate the potential effect of salidroside on osteoclasts.
RESULTS
SAL enhanced the expression of HIF-1 and its downstream target genes in osteoclasts. Immunofluorescence analysis confirmed the facilitation of HIF-1 nuclear translocation and transcriptional activity by SAL. In addition, SAL enhanced osteoclast viability, differentiation and bone resorption activity in an autocrine manner through HIF-1/VEGF, IL-6 and ANGPTL4 pathways.
CONCLUSION
SAL promotes osteoclast proliferation, differentiation and bone resorption through HIF-1/VEGF, IL-6 and ANGPTL4 pathways.
背景与目的
本研究旨在通过调节 HIF-1 通路及其下游靶基因，探讨红景天苷（SAL）（红景天的主要活性化合物）在调节破骨细胞分化和功能方面的潜力。
方法
用 SAL 处理破骨细胞后，在 mRNA 和蛋白质水平上检测 HIF-1 及其下游靶基因的表达。免疫荧光分析用于评估 SAL 对 HIF-1 核易位和转录活性的影响。MTT、流式细胞术、qPCR、TRAP 染色和骨吸收测定用于评估 SAL 对破骨细胞的潜在影响。
结果
SAL 增强了破骨细胞中 HIF-1 及其下游靶基因的表达。免疫荧光分析证实了 SAL 促进 HIF-1 核易位和转录活性。此外，SAL 通过 HIF-1/VEGF、IL-6 和 ANGPTL4 途径以自分泌方式增强破骨细胞的活力、分化和骨吸收活性。
结论
SAL 通过 HIF-1/VEGF、IL-6 和 ANGPTL4 途径促进破骨细胞增殖、分化和骨吸收。
Zotero 插件