Laboratory of Molecular and Systemic Neuromorphology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warsaw, Poland.
Laboratory of Cell Biophysics, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warsaw, Poland.
Biochim Biophys Acta Mol Cell Res. 2024 Dec;1871(8):119829. doi: 10.1016/j.bbamcr.2024.119829. Epub 2024 Aug 26.
Nuclear speckles, also known as interchromatin granule clusters (IGCs), are subnuclear domains highly enriched in proteins involved in transcription and mRNA metabolism and, until recently, have been regarded primarily as their storage and modification hubs. However, several recent studies on non-neuronal cell types indicate that nuclear speckles may directly contribute to gene expression as some of the active genes have been shown to associate with these structures. Neuronal activity is one of the key transcriptional regulators and may lead to the rearrangement of some nuclear bodies. Notably, the impact of neuronal activation on IGC/nuclear speckles organization and function remains unexplored. To address this research gap, we examined whether and how neuronal stimulation affects the organization of these bodies in granular neurons from the rat hippocampal formation. Our findings demonstrate that neuronal stimulation induces morphological and proteomic remodelling of the nuclear speckles under both in vitro and in vivo conditions. Importantly, these changes are not associated with cellular stress or cell death but are dependent on transcription and splicing.
核斑点，也称为染色质间颗粒簇（IGCs），是富含参与转录和 mRNA 代谢的蛋白质的亚核域，直到最近，它们主要被视为其储存和修饰中心。然而，最近对非神经元细胞类型的几项研究表明，核斑点可能直接有助于基因表达，因为已经表明一些活性基因与这些结构相关联。神经元活动是关键的转录调节因子之一，可能导致一些核体的重新排列。值得注意的是，神经元激活对 IGC/核斑点组织和功能的影响仍未得到探索。为了解决这一研究空白，我们研究了神经元刺激是否以及如何影响来自大鼠海马结构的颗粒神经元中这些体的组织。我们的研究结果表明，神经元刺激在体外和体内条件下诱导核斑点的形态和蛋白质组重塑。重要的是，这些变化与细胞应激或细胞死亡无关，而是依赖于转录和剪接。
