Molecular Genetics Laboratory, Institute of Plant Biology and Biotechnology, Almaty 050040, Kazakhstan.
Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan.
Biomolecules. 2024 Jul 24;14(8):890. doi: 10.3390/biom14080890.
The taxonomic classification of the genera L., Akhani and Roalson, and Tzvelev within Chenopodiaceae Vent. (Amaranthaceae s.l.) remains controversial, with the precise number of species within these genera still unresolved. This study presents a comparative analysis of the complete plastid genomes of , , , and species collected in Kazakhstan. The assembled plastid genomes varied in length, ranging from 151,177 bp to 152,969 bp for and . These genomes contained 133 genes, of which 114 were unique, including 80 protein-coding, 30 tRNA, and 4 rRNA genes. Thirteen regions, including , , , , , , , , , , , , and , exhibited relatively high levels of nucleotide variation. A total of 987 SSRs were detected across the four analyzed plastid genomes, primarily located in the intergenic spacer regions. Additionally, 254 repeats were identified, including 92 tandem repeats, 88 forward repeats, 100 palindromic repeats, and only one reverse repeat. A phylogenetic analysis revealed clear clustering into four clusters corresponding to the Salsoleae and Caroxyloneae tribe clades. These nucleotide sequences obtained in this study represent a valuable resource for future phylogenetic analyses within the Salsoleae s.l. tribe.
Chenopodiaceae Vent.（苋科）中的 L.、Akhani 和 Roalson 以及 Tzvelev 属的分类仍然存在争议，这些属内的确切物种数量仍未解决。本研究对在哈萨克斯坦收集的 、 、 和 种的完整质体基因组进行了比较分析。组装的质体基因组在长度上有所不同， 和 的长度范围分别为 151,177 bp 到 152,969 bp。这些基因组包含 133 个基因，其中 114 个是独特的，包括 80 个蛋白编码基因、30 个 tRNA 和 4 个 rRNA 基因。13 个区域，包括 、 、 、 、 、 、 、 、 、 、和 ，表现出相对较高的核苷酸变异水平。在四个分析的质体基因组中总共检测到 987 个 SSR，主要位于基因间间隔区。此外，还鉴定出 254 个重复，包括 92 个串联重复、88 个正向重复、100 个回文重复和仅 1 个反向重复。系统发育分析显示，它们清晰地聚类为四个簇，对应于 Salsoleae 和 Caroxyloneae 族聚类。本研究获得的这些核苷酸序列为 Salsoleae s.l.族的未来系统发育分析提供了有价值的资源。
