Schiffers Christina, Serbetci Idil, Mense Kirsten, Kassens Ana, Grothmann Hanna, Sommer Matthias, Hoeflich Christine, Hoeflich Andreas, Bollwein Heinrich, Schmicke Marion
Veterinary-Endocrinology and Laboratory Diagnostics, Clinic for Cattle, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany.
Clinic of Reproductive Medicine, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland.
Animals (Basel). 2024 Aug 15;14(16):2370. doi: 10.3390/ani14162370.
Insulin-like growth factor 1 (IGF-1) regulates dairy cow reproduction, while the paracrine IGF system locally influences fertility. In both systems, IGF-1 bioactivity is regulated through binding proteins (IGFBPs) inhibiting IGF-1 binding to its receptor (IGF1R). This study aimed to investigate a possible transfer between this endocrine and paracrine system. Therefore, blood and follicular fluid (FF) from postpartum dairy cows were analysed for ß-hydroxybutyrate (BHB), IGF-1, IGFBP-2, -3, -4, -5, and an IGFBP fragment in two study parts. The mRNA expression of , , , and the pregnancy-associated plasma protein A () in granulosa cells was measured. The results showed correlations between plasma and FF for IGF-1 (r = 0.57, < 0.001) and IGFBP-2 (r = -0.57, < 0.05). Blood BHB negatively correlated with IGF-1 in blood and FF and IGFBP-3, -5 and total IGFBP in blood (IGF-1 plasma: r = -0.26, < 0.05; FF: r = -0.35, < 0.05; IGFBP-3: r = -0.64, = 0.006; IGFBP-5: r = -0.49, < 0.05; total IGFBP: r = -0.52, < 0.05). A negative correlation was found between expression and IGF-1 concentration in FF (r = -0.97, = 0.001), while an IGFBP fragment positively correlated with -mRNA in FF (r = 0.82, = 0.042). These findings suggest a transfer and local regulation between the somatotropic axis and the follicular IGF system, linking the metabolic status with local effects on dairy cow fertility.
胰岛素样生长因子1（IGF-1）调节奶牛繁殖，而旁分泌IGF系统局部影响生育能力。在这两个系统中，IGF-1生物活性通过结合蛋白（IGFBPs）抑制IGF-1与其受体（IGF1R）结合来调节。本研究旨在调查该内分泌和旁分泌系统之间可能的转移。因此，在两个研究部分中，对产后奶牛的血液和卵泡液（FF）进行了β-羟基丁酸（BHB）、IGF-1、IGFBP-2、-3、-4、-5以及一种IGFBP片段的分析。测量了颗粒细胞中、、和妊娠相关血浆蛋白A（）的mRNA表达。结果显示，血浆和FF中的IGF-1（r = 0.57，< 0.001）和IGFBP-2（r = -0.57，< 0.05）之间存在相关性。血液BHB与血液和FF中的IGF-1以及血液中的IGFBP-3、-5和总IGFBP呈负相关（IGF-1血浆：r = -0.26，< 0.05；FF：r = -0.35，< 0.05；IGFBP-3：r = -0.64，= 0.006；IGFBP-5：r = -0.49，< 0.05；总IGFBP：r = -0.52，< 0.05）。发现FF中表达与IGF-1浓度呈负相关（r = -0.97，= 0.001），而FF中的一个IGFBP片段与FF中的-mRNA呈正相关（r = 0.82，= 0.042）。这些发现表明生长激素轴和卵泡IGF系统之间存在转移和局部调节，将代谢状态与对奶牛生育能力的局部影响联系起来。
