Tran Phat L, Presson Caroline L, Kashem Md Nayeem Hasan, Li Wei, Reid Ted W, de Riese Werner T W
Department of Ophthalmology and Visual Sciences, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA.
Department of Urology, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA.
Antibiotics (Basel). 2024 Aug 6;13(8):736. doi: 10.3390/antibiotics13080736.
To further investigate the inhibition of 's in vitro growth and biofilm formation by an organo-selenium-incorporated polyurethane (PU) catheter material. , , and were incubated in vitro with organo-selenium and control polyurethane catheter materials in the presence of glutathione. Growth was evaluated by a colony-forming-unit (CFU) count and visualized with confocal laser scanning microscopy. Two different PU catheter materials were used. Using tin-catalyzed PU catheter material, complete inhibition of was seen at 1% selenium (Se), whereas no inhibition was seen for at up to 3.0% Se. Whereas, using a thermoplastic PU catheter material, 1.5% Se and 2% Se organo-selenium caused several logs of growth inhibition of , and 2.5% selenium, incorporation showed complete inhibition (8 logs). Samples with lower than 1.5% selenium did not show adequate growth inhibition for . Similar in vitro growth inhibition was achieved against a multidrug-resistant strain. It was concluded that optimal inhibition of in vitro growth and biofilm formation occurs with 2.5% selenium incorporated as organo-selenium in a thermoplastic PU catheter material. These results suggest that reduced incidence of CAUTIs (catheter associated urinary tract infections) with and other bacteria and fungi can be achieved by using organo-selenium-incorporated catheters.
为进一步研究含有机硒的聚氨酯（PU）导管材料对[细菌名称]体外生长和生物膜形成的抑制作用。将[细菌名称]在谷胱甘肽存在的情况下与有机硒和对照聚氨酯导管材料进行体外培养。通过菌落形成单位（CFU）计数评估生长情况，并用共聚焦激光扫描显微镜进行可视化观察。使用了两种不同的PU导管材料。使用锡催化的PU导管材料时，在硒（Se）含量为1%时可观察到对[细菌名称]的完全抑制，而在硒含量高达3.0%时对[细菌名称]未观察到抑制作用。然而，使用热塑性PU导管材料时，1.5% Se和2% Se的有机硒导致[细菌名称]的生长抑制达几个对数级，而2.5%硒的掺入显示出完全抑制（8个对数级）。硒含量低于1.5%的样品对[细菌名称]未显示出足够的生长抑制作用。对一株耐多药[细菌名称]菌株也实现了类似的体外生长抑制。得出的结论是，在热塑性PU导管材料中以有机硒形式掺入2.5%的硒可实现对[细菌名称]体外生长和生物膜形成的最佳抑制。这些结果表明，使用含有机硒的导管可降低由[细菌名称]及其他细菌和真菌引起的导管相关尿路感染（CAUTIs）的发生率。
