Rosberg Anna Karin, Silva Maria João, Skøtt Feidenhans'l Cecilie, Cytryn Eddie, Jurkevitch Edouard, Lood Rolf
Microbial Horticulture Laboratory, Department of Biosystems and Technology, Swedish University of Agricultural Sciences, 234 56 Alnarp, Sweden.
Department of Clinical Sciences Lund, Division of Infection Medicine, Lund University, 221 84 Lund, Sweden.
Antibiotics (Basel). 2024 Aug 10;13(8):750. doi: 10.3390/antibiotics13080750.
Antibiotic resistance genes (ARGs) are widespread in the environment, and soils, specifically, are hotspots for microorganisms with inherent antibiotic resistance. Manure and sludge used as fertilizers in agricultural production have been shown to contain vast amounts of ARGs, and due to continued applications, ARGs accumulate in agricultural soils. Some soils, however, harbor a resilience capacity that could depend on specific soil properties, as well as the presence of predatory bacteria that are able to hydrolyse living bacteria, including bacteria of clinical importance. The objectives of this study were to (i) investigate if the antibiotic resistance profile of the soil microbiota could be differently affected by the addition of cow manure, chicken manure, and sludge, and (ii) investigate if the amendments had an effect on the presence of predatory bacteria. The three organic amendments were mixed separately with a field soil, divided into pots, and incubated in a greenhouse for 28 days. Droplet digital PCR (ddPCR) was used to quantify three ARGs, two predatory bacteria, and total number of bacteria. In this study, we demonstrated that the choice of organic amendment significantly affected the antibiotic resistance profile of soil, and promoted the growth of predatory bacteria, while the total number of bacteria was unaffected.
抗生素抗性基因（ARGs）在环境中广泛存在，特别是土壤，是具有固有抗生素抗性的微生物的热点区域。农业生产中用作肥料的粪便和污泥已被证明含有大量的ARGs，并且由于持续施用，ARGs在农业土壤中积累。然而，一些土壤具有恢复能力，这可能取决于特定的土壤性质，以及能够水解活细菌（包括具有临床重要性的细菌）的捕食性细菌的存在。本研究的目的是：（i）研究添加牛粪、鸡粪和污泥是否会对土壤微生物群的抗生素抗性谱产生不同影响；（ii）研究这些改良剂是否对捕食性细菌的存在有影响。将这三种有机改良剂分别与田间土壤混合，装入花盆，在温室中培养28天。采用液滴数字PCR（ddPCR）定量三种ARGs、两种捕食性细菌和细菌总数。在本研究中，我们证明了有机改良剂的选择显著影响土壤的抗生素抗性谱，并促进捕食性细菌的生长，而细菌总数不受影响。
