Gallego-Barceló Paula, Benítez-Álvarez David, Bagues Ana, Silván-Ros Blanca, Montalbán-Rodríguez Alba, López-Gómez Laura, Vera Gema, Del Castillo María Dolores, Uranga José A, Abalo Raquel
Department of Basic Health Sciences, Faculty of Health Sciences, University Rey Juan Carlos (URJC), 28922 Alcorcón, Spain.
High Performance Research Group in Physiopathology and Pharmacology of the Digestive System (NeuGut-URJC), University Rey Juan Carlos, 28922 Alcorcón, Spain.
Foods. 2024 Aug 6;13(16):2474. doi: 10.3390/foods13162474.
Instant Cascara (IC) is a sustainable beverage made from dried coffee cherry pulp, a by-product of coffee processing. It is rich in nutrients and bioactive compounds and has a high concentration of antioxidants. This study explored the impact of regular IC consumption on colonic motor function and innervation. Over a period of 4 weeks, male and female healthy rats were given drinking water containing 10 mg/mL of IC. Thereafter, colon samples were obtained to evaluate the longitudinal (LM) and circular (CM) smooth muscle contractile response to acetylcholine (ACh) and electrical field stimulation (EFS) in an organ bath, before and after atropine administration (10 M). Histological and immunohistochemical analyses assessed colon damage, muscle thickness, and immunoreactivity to substance P (SP) and neuronal nitric oxide synthase (nNOS). ACh and EFS induced similar responses across groups, but the CM response to EFS was greater in females compared with males, despite their lower body weight. Atropine completely blocked the response to ACh but only partially antagonized the neural response to EFS, particularly that of CM in females treated with IC, which had a greater liquid intake than those exposed to water. However, in the myenteric ganglia, no statistically significant differences were observed in SP or nNOS. Our results suggest that regular IC exposure may enhance specific neural pathway functions, particularly in females, possibly due to their increased IC consumption.
速溶卡斯卡拉（IC）是一种由咖啡加工副产品——干燥咖啡樱桃果肉制成的可持续饮品。它富含营养和生物活性化合物，且抗氧化剂浓度很高。本研究探讨了定期饮用IC对结肠运动功能和神经支配的影响。在4周的时间里，给雄性和雌性健康大鼠饮用含有10毫克/毫升IC的饮用水。此后，获取结肠样本，以评估在给予阿托品（10微摩尔）前后，离体器官浴中结肠纵行（LM）和环行（CM）平滑肌对乙酰胆碱（ACh）和电场刺激（EFS）的收缩反应。组织学和免疫组织化学分析评估结肠损伤、肌肉厚度以及对P物质（SP）和神经元型一氧化氮合酶（nNOS）的免疫反应性。ACh和EFS在各实验组中诱导出相似的反应，但尽管雌性大鼠体重较低，其CM对EFS的反应却比雄性大鼠更强。阿托品完全阻断了对ACh的反应，但仅部分拮抗了对EFS的神经反应，特别是在饮用IC的雌性大鼠中CM的反应，这些雌性大鼠的液体摄入量比饮用清水的大鼠更多。然而，在肌间神经节中，未观察到SP或nNOS有统计学上的显著差异。我们的结果表明，定期接触IC可能会增强特定神经通路的功能，尤其是在雌性大鼠中，这可能是由于它们对IC的摄入量增加所致。
