Hua Zi, Zhu Mei-Jun
School of Food Science, Washington State University, Pullman, WA 99164, USA.
Foods. 2024 Aug 7;13(16):2481. doi: 10.3390/foods13162481.
The persistence of biofilms on equipment surfaces poses a significant risk of cross-contamination, necessitating effective surface decontamination strategies. This study assessed the effectiveness of hurdle treatments combining peroxyacetic acid (PAA) and saturated steam against 7-day-old (a non-pathogenic surrogate for ) biofilms on stainless steel (SS), polyester (PET), and rubber surfaces. Results demonstrated >6 log CFU/coupon reductions on SS and PET surfaces after PAA (40 ppm, 1 min) followed by steam treatment (100 °C, 6 s). On rubber surfaces, PAA (80 ppm, 1 min) followed by steam treatment (100 °C, 6 s) resulted in ~5 log CFU/coupon reduction. The presence of apple juice soil reduced the efficacy of hurdle treatments, with PAA (40 ppm, 1 min) and steam exposure (6 s) resulting in 5.6, 5.8, and 4.2 log CFU/coupon reductions of on SS, PET, and rubber, respectively. The efficacy of this antimicrobial combination was further reduced by surface defects, especially in the presence of organic matter. Nevertheless, the treatment still achieved >5 log CFU/coupon reductions of on worn SS and PET soiled with apple juice and ~4.5 log CFU/coupon reduction on worn, soiled rubber surfaces. These findings highlight that PAA treatments followed by a brief steam exposure are effective strategies for controlling on food-contact surfaces.
生物膜在设备表面的持续存在带来了显著的交叉污染风险，因此需要有效的表面去污策略。本研究评估了过氧乙酸（PAA）和饱和蒸汽联合障碍处理对不锈钢（SS）、聚酯（PET）和橡胶表面上7日龄（一种非致病性替代物）生物膜的有效性。结果表明，在PAA（40 ppm，1分钟）处理后进行蒸汽处理（100°C，6秒），SS和PET表面的菌落形成单位（CFU）/试片减少了>6 log。在橡胶表面，PAA（80 ppm，1分钟）后进行蒸汽处理（100°C，6秒）导致CFU/试片减少约5 log。苹果汁污垢的存在降低了障碍处理的效果，PAA（40 ppm，1分钟）和蒸汽暴露（6秒）分别使SS、PET和橡胶表面的CFU/试片减少了5.6、5.8和4.2 log。表面缺陷进一步降低了这种抗菌组合的效果，尤其是在存在有机物的情况下。尽管如此，该处理仍使沾有苹果汁的磨损SS和PET表面的CFU/试片减少>5 log，使磨损且沾污的橡胶表面的CFU/试片减少约4.5 log。这些发现突出表明，PAA处理后进行短暂的蒸汽暴露是控制食品接触表面生物膜的有效策略。
