Liu Huanming, Huang Ailian, Yi Jiawen, Luo Meiyan, Jiang Guili, Guan Jingjing, Liu Shucheng, Deng Chujin, Luo Donghui
College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China.
Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Zhanjiang 524088, China.
Foods. 2024 Aug 13;13(16):2523. doi: 10.3390/foods13162523.
This study was conducted to investigate the effect of inoculation with FELA1 isolated from traditional shrimp paste and koji on the taste, flavor characteristics, and bacterial community of rapidly fermented shrimp paste. E-nose and e-tongue results showed higher levels of alcohols, aldehydes, and ketones, enhanced umami and richness, and reduced bitterness and astringency in samples of shrimp paste inoculated with fermentation ( < 0.05). Eighty-two volatile compounds were determined using headspace solid-phase microextraction and gas chromatography-mass spectrometry (HS-SPEM-GC-MS). The contents of 3-methyl-1-butanol, phenylethanol, isovaleraldehyde, and 2-nonanone in the inoculated samples were significantly increased ( < 0.05), resulting in pleasant odors such as almond, floral, and fruity. High-throughput sequencing results showed that the addition of koji and FELA1 changed the composition and abundance of bacteria and reduced the abundance of harmful bacteria. Spearman's correlation coefficient indicated that the alcohols, aldehydes, and ketones of the inoculated fermented samples showed a strong correlation (|ρ| > 0.6) with and , which contributed to the formation of good flavor in the fast fermented shrimp paste. This study may offer new insights into the production of rapidly fermented shrimp paste with better taste and flavor.
本研究旨在探讨接种从传统虾酱和曲中分离出的FELA1对快速发酵虾酱的口感、风味特征和细菌群落的影响。电子鼻和电子舌结果表明,接种发酵的虾酱样品中醇类、醛类和酮类含量较高,鲜味和丰富度增强,苦味和涩味降低(<0.05)。采用顶空固相微萃取和气相色谱-质谱联用(HS-SPEM-GC-MS)测定了82种挥发性化合物。接种样品中3-甲基-1-丁醇、苯乙醇、异戊醛和2-壬酮的含量显著增加(<0.05),产生了杏仁、花香和果香等宜人气味。高通量测序结果表明,添加曲和FELA1改变了细菌的组成和丰度,降低了有害细菌的丰度。Spearman相关系数表明,接种发酵样品中的醇类、醛类和酮类与和呈强相关性(|ρ|>0.6),这有助于快速发酵虾酱中良好风味的形成。本研究可能为生产口感和风味更好的快速发酵虾酱提供新的见解。