Stanca Loredana, Bilteanu Liviu, Bujor Oana Crina, Ion Violeta Alexandra, Petre Andrei Cătălin, Bădulescu Liliana, Geicu Ovidiu Ionut, Pisoschi Aurelia Magdalena, Serban Andreea Iren, Ghimpeteanu Oana-Mărgărita
Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine Bucharest, 105 Blvd, Splaiul Independenței, 050097 Bucharest, Romania.
Research Center for Studies of Food Quality and Agricultural Products, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Mărăşti Blvb, 011464 Bucharest, Romania.
Foods. 2024 Aug 16;13(16):2552. doi: 10.3390/foods13162552.
Developing of functional foods is a promising strategy to reduce the increasing burden of colorectal cancer worldwide. Fruit pomace, particularly polyphenol and anthocyanin-rich chokeberry and blueberry, is a valuable ingredient for functional foods and nutraceuticals. Our study aimed to evaluate the anti-inflammatory and antiproliferative effects of chokeberry and blueberry pomace extracts on C2BBe1 colorectal carcinoma cells and explore the underlying signaling pathways. We analyzed both pomace extracts for total polyphenols and anthocyanins using Folin-Ciocalteu method and ultra-performance liquid chromatography, while antioxidative activity was assessed via the 2,2-diphenyl-1-picrylhydrazyl radical scavenging method. We evaluated the in vitro anti-inflammatory and antiproliferative effects using trypan blue exclusion, MTT and LDH assays, and assessed protein levels of p-Erk1/2, Akt-1, STAT1, STAT3, occludin, oxidized proteins, and MDA-protein adducts through western blotting, as well as analysis of a 37-plex panel of inflammatory markers. Chokeberry extracts exhibited higher total polyphenol content, anthocyanin levels, and antioxidative activity compared to blueberry extracts, however, blueberry extracts effects on cell viability and proliferation in C2BBe1 cells were stronger. Both fruit pomaces induced non-inflammatory cell death characterized by membrane integrity loss, beneficial in cancer therapy. Our data suggests chokeberry's cytotoxicity may be mediated by Erk signaling and Akt-1 inhibition, while blueberry uniquely decreased occludin levels. These berries pomaces' potential to mitigate cancer risks and enhance treatment efficacy is promising, warranting further investigation for functional foods development.
开发功能性食品是减轻全球结直肠癌日益加重负担的一项有前景的策略。水果渣，特别是富含多酚和花青素的黑果腺肋花楸和蓝莓，是功能性食品和营养保健品的宝贵成分。我们的研究旨在评估黑果腺肋花楸和蓝莓渣提取物对C2BBe1结肠癌细胞的抗炎和抗增殖作用，并探索其潜在的信号通路。我们使用Folin-Ciocalteu法和超高效液相色谱法分析了两种果渣提取物中的总多酚和花青素，同时通过2,2-二苯基-1-苦基肼自由基清除法评估了抗氧化活性。我们使用台盼蓝排斥法、MTT法和LDH法评估了体外抗炎和抗增殖作用，并通过蛋白质印迹法评估了p-Erk1/2、Akt-1、STAT1、STAT3、闭合蛋白、氧化蛋白和MDA-蛋白加合物的蛋白质水平，以及分析了一组37种炎症标志物。与蓝莓提取物相比，黑果腺肋花楸提取物表现出更高的总多酚含量、花青素水平和抗氧化活性，然而，蓝莓提取物对C2BBe1细胞活力和增殖的影响更强。两种水果渣均诱导了以膜完整性丧失为特征的非炎性细胞死亡，这对癌症治疗有益。我们的数据表明，黑果腺肋花楸的细胞毒性可能由Erk信号传导和Akt-1抑制介导，而蓝莓则独特地降低了闭合蛋白水平。这些浆果渣减轻癌症风险和提高治疗效果有潜力，值得进一步研究以开发功能性食品。
