Janowsky A, Schweri M M, Berger P, Long R, Skolnick P, Paul S M
Eur J Pharmacol. 1985 Jan 22;108(2):187-91. doi: 10.1016/0014-2999(85)90724-1.
The specific binding of [3H]threo-(+/-)-methylphenidate to membranes prepared from rat striatum was significantly reduced following either surgical lesions of the medial forebrain bundle or intracerebroventricular administration of 6-hydroxydopamine. The decrease in the density of [3H]threo-(+/-)-methylphenidate binding sites in striatum following chemical or surgical denervation was highly correlated with the decrease in [3H]dopamine uptake. In contrast, intracerebroventricular administration of 5,7-dihydroxytryptamine, AF64A, or chronic parenteral administration of reserpine did not alter either the number of apparent affinity of [3H]threo-(+/-)-methylphenidate binding sites. These data suggest that the specific binding sites for [3H]-threo-(+/-)-methylphenidate in striatum are localized to dopaminergic nerve terminals, and may be associated with the dopamine transport complex.
内侧前脑束进行手术损伤或脑室内注射6-羟基多巴胺后，[3H]苏式-（+/-）-哌醋甲酯与大鼠纹状体制备的膜的特异性结合显著降低。化学或手术去神经支配后纹状体中[3H]苏式-（+/-）-哌醋甲酯结合位点密度的降低与[3H]多巴胺摄取的降低高度相关。相比之下，脑室内注射5,7-二羟基色胺、AF64A或长期肠胃外注射利血平，均未改变[3H]苏式-（+/-）-哌醋甲酯结合位点的表观亲和力数量。这些数据表明，纹状体中[3H] - 苏式-（+/-）-哌醋甲酯的特异性结合位点定位于多巴胺能神经末梢，并且可能与多巴胺转运复合体相关。
