Geisler N, Plessmann U, Weber K
FEBS Lett. 1985 Mar 25;182(2):475-8. doi: 10.1016/0014-5793(85)80357-4.
The first complete amino acid sequence of a neurofilament protein has been established. Porcine NF-L contains 548 residues corresponding to a molecular mass of approximately 62 kDa. This value is noticeably smaller than the 68-72 kDa estimates from gel electrophoresis. Sequence comparison among the 6 non-epithelial intermediate filament (IF) proteins of warm-blooded vertebrates shows that the three NF proteins are the most remote members. Additionally and unexpectedly they reveal among each other lower sequence identity than the three non-neuronal IF proteins GFAP, desmin, and vimentin where the last two are particularly closely related. Certain schemes of IF protein evolution are discussed.
神经丝蛋白的首个完整氨基酸序列已被确定。猪的神经丝轻链蛋白（NF-L）含有548个残基，其分子量约为62 kDa。该值明显小于凝胶电泳估计的68 - 72 kDa。对温血脊椎动物的6种非上皮中间丝（IF）蛋白进行序列比较表明，三种神经丝蛋白是关系最疏远的成员。此外，出乎意料的是，它们彼此之间的序列同一性低于三种非神经元中间丝蛋白，即胶质纤维酸性蛋白（GFAP）、结蛋白和波形蛋白，其中后两者的关系尤为密切。文中讨论了中间丝蛋白进化的某些模式。
