Zeiner Sebastian, Wohlrab Peter, Rosicky Ingo, Schukro Regina Patricia, Klein Klaus Ulrich, Wojta Johann, Speidl Walter, Kiss Herbert, Muin Dana Anaïs
Department of Anaesthesia, Intensive Care Medicine and Pain Medicine, Medical University of Vienna, 1090 Vienna, Austria.
Department of Obstetrics and Gynaecology, Division of Obstetrics and Feto-Maternal Medicine, Medical University of Vienna, 1090 Vienna, Austria.
J Clin Med. 2024 Aug 7;13(16):4616. doi: 10.3390/jcm13164616.
: Intra-amniotic infection (IAI) is a rare but serious condition with potential complications such as preterm labor and intrauterine fetal death. Diagnosing IAI is challenging due to varied clinical signs. Oxidative stress and mitochondrial dysfunction have been hypothesized to evolve around IAI. This study focused on measuring circulating mtDNA levels, a proposed biomarker for mitochondrial dysfunction, in maternal serum and placenta of women with confirmed IAI and healthy controls. : 12 women with confirmed IAI (IAI group) were enrolled following premature preterm rupture of the membranes (PPROM) and compared to 21 healthy women (control group). Maternal blood was obtained two weeks pre-partum and peripartum; furthermore, postpartum placental blood was taken. In the IAI group, maternal blood was taken once weekly until delivery as well as peripartum, as was placental blood. Circulating cell-free mtDNA was quantified by real-time quantitative PCR. : Upon admission, in the IAI group, mean plasma mtDNA levels were 735.8 fg/μL compared to 134.0 fg/μL in the control group ( < 0.05). After delivery, in the IAI group, mean mtDNA levels in the placenta were 3010 fg/μL versus 652.4 fg/μL ( < 0.05). : Circulating cell-free mtDNA could serve as a valuable biomarker for IAI prediction and diagnosis. Future research should establish reference values for sensitivity in predicting IAI.
羊膜腔内感染（IAI）是一种罕见但严重的疾病，可能会引发早产和胎儿宫内死亡等并发症。由于临床症状多样，IAI的诊断具有挑战性。氧化应激和线粒体功能障碍被认为与IAI的发生发展有关。本研究聚焦于测量确诊为IAI的女性及健康对照者的母血和胎盘中循环线粒体DNA（mtDNA）水平，mtDNA是一种被认为可用于评估线粒体功能障碍的生物标志物。12例确诊为IAI的女性（IAI组）在胎膜早破（PPROM）后入组，并与21例健康女性（对照组）进行比较。在产前两周、围产期采集母血；此外，产后采集胎盘血。在IAI组，每周采集一次母血直至分娩以及围产期均采集，胎盘血也是如此。通过实时定量PCR对循环游离mtDNA进行定量分析。入院时，IAI组血浆mtDNA平均水平为735.8 fg/μL，而对照组为134.0 fg/μL（P<0.05）。分娩后，IAI组胎盘mtDNA平均水平为3010 fg/μL，而对照组为652.4 fg/μL（P<0.05）。循环游离mtDNA可作为预测和诊断IAI的有价值生物标志物。未来研究应确定预测IAI时敏感性的参考值。
