School of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, China.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.
Int J Mol Sci. 2024 Aug 10;25(16):8726. doi: 10.3390/ijms25168726.
High-Protein Mulberry is a novel strain of mulberry. High-Protein Mulberry leaves (HPM) were the subject of this study, which aimed to investigate its efficacy and underlying mechanisms in modulating glucose and lipid metabolism. A six-week intervention using / mice was carried out to assess the effects of HPM on serum lipid levels, liver function, and insulin (INS) levels. qRT-PCR and Western Blotting were employed to measure key RNA and protein expressions in the PI3K/Akt and PPARα/CPT-1 pathways. UHPLC-MS and the Kjeldahl method were utilized to analyze the component content and total protein. Additionally, network pharmacology was employed to predict regulatory mechanism differences between HPM and Traditional Mulberry leaves. The results of the study revealed significant improvements in fasting blood glucose, glucose tolerance, and insulin resistance in mice treated with HPM. HPM notably reduced serum levels of total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and INS, while increasing high-density lipoprotein cholesterol (HDL-C) levels. The treatment also effectively mitigated liver fatty lesions, inflammatory infiltration, and islet atrophy. HPM activation of the PI3K/Akt/PPARα/CPT-1 pathway suggested its pivotal role in the regulation of glucose and lipid metabolism. With its rich composition and pharmacodynamic material basis, HPM displayed a greater number of targets associated with glucose and lipid metabolism pathways, underscoring the need for further research into its potential therapeutic applications.
高蛋白桑是一种新型的桑树品种。本研究以高蛋白桑叶（HPM）为研究对象，旨在探讨其调节糖脂代谢的功效及作用机制。通过对 / 小鼠进行为期 6 周的干预实验，评估 HPM 对血清脂质水平、肝功能和胰岛素（INS）水平的影响。采用 qRT-PCR 和 Western Blotting 技术测定 PI3K/Akt 和 PPARα/CPT-1 通路中关键 RNA 和蛋白的表达水平。采用 UHPLC-MS 和凯氏定氮法分析其成分含量和总蛋白。此外，还运用网络药理学预测 HPM 与传统桑树叶之间调控机制的差异。研究结果表明，HPM 可显著改善小鼠的空腹血糖、葡萄糖耐量和胰岛素抵抗。HPM 可明显降低血清总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）、天门冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）和 INS 水平，同时升高高密度脂蛋白胆固醇（HDL-C）水平。该治疗还可有效减轻肝脂肪病变、炎症浸润和胰岛萎缩。HPM 通过激活 PI3K/Akt/PPARα/CPT-1 通路，提示其在调节糖脂代谢中发挥关键作用。HPM 具有丰富的成分和药效物质基础，与糖脂代谢途径相关的靶点数量更多，这表明需要进一步研究其在治疗中的潜在应用。
