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过表达神经黏连蛋白和神经连接蛋白的人神经干细胞对脊髓损伤模型的影响。

Effects of Human Neural Stem Cells Overexpressing Neuroligin and Neurexin in a Spinal Cord Injury Model.

机构信息

Department of Biology Education, Korea National University of Education, Cheongju 28173, Chungbuk, Republic of Korea.

Department of Life Sports Educator, Kongju National University, Kongju 32588, Chungnam, Republic of Korea.

出版信息

Int J Mol Sci. 2024 Aug 10;25(16):8744. doi: 10.3390/ijms25168744.

DOI:10.3390/ijms25168744
PMID:39201431
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11354780/
Abstract

Recent studies have highlighted the therapeutic potential of stem cells for various diseases. However, unlike other tissues, brain tissue has a specific structure, consisting of synapses. These synapses not only transmit but also process and refine information. Therefore, synaptic regeneration plays a key role in therapy of neurodegenerative disorders. Neurexins (NRXNs) and neuroligins (NLGNs) are synaptic cell adhesion molecules that connect pre- and postsynaptic neurons at synapses, mediate trans-synaptic signaling, and shape neural network properties by specifying synaptic functions. In this study, we investigated the synaptic regeneration effect of human neural stem cells (NSCs) overexpressing NRXNs (F3.NRXN) and NLGNs (F3.NLGN) in a spinal cord injury model. Overexpression of NRXNs and NLGNs in the neural stem cells upregulated the expression of synaptophysin, PSD95, VAMP2, and synapsin, which are synaptic markers. The BMS scores indicated that the transplantation of F3.NRXN and F3.NLGN enhanced the recovery of locomotor function in adult rodents following spinal cord injury. Transplanted F3.NRXN and F3.NLGN differentiated into neurons and formed a synapse with the host cells in the spinal cord injury mouse model. In addition, F3.NRXN and F3.NLGN cells restored growth factors (GFs) and neurotrophic factors (NFs) and induced the proliferation of host cells. This study suggested that NSCs overexpressing NRXNs and NLGNs could be candidates for cell therapy in spinal cord injuries by facilitating synaptic regeneration.

摘要

最近的研究强调了干细胞在各种疾病治疗中的潜力。然而，与其他组织不同，脑组织具有特定的结构，由突触组成。这些突触不仅传递信息，还处理和精炼信息。因此，突触再生在神经退行性疾病的治疗中起着关键作用。神经连接蛋白（NRXNs）和神经黏附素（NLGNs）是突触细胞粘附分子，它们在突触处连接前突触和后突触神经元，介导跨突触信号传递，并通过指定突触功能来塑造神经网络特性。在这项研究中，我们研究了过表达 NRXNs（F3.NRXN）和 NLGNs（F3.NLGN）的人神经干细胞（NSCs）在脊髓损伤模型中的突触再生作用。NRXNs 和 NLGNs 在神经干细胞中的过表达上调了突触蛋白、PSD95、VAMP2 和突触小体的表达，这些都是突触标记物。BMS 评分表明，F3.NRXN 和 F3.NLGN 的移植增强了成年啮齿动物脊髓损伤后运动功能的恢复。移植的 F3.NRXN 和 F3.NLGN 在脊髓损伤小鼠模型中分化为神经元，并与宿主细胞形成突触。此外，F3.NRXN 和 F3.NLGN 细胞恢复了生长因子（GFs）和神经营养因子（NFs），并诱导宿主细胞的增殖。这项研究表明，过表达 NRXNs 和 NLGNs 的 NSCs 可以通过促进突触再生成为脊髓损伤细胞治疗的候选者。

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本文引用的文献

1
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Int J Mol Sci. 2023 Sep 20;24(18):14349. doi: 10.3390/ijms241814349.
2
Neuroplasticity and regeneration after spinal cord injury.脊髓损伤后的神经可塑性与再生
N Am Spine Soc J. 2023 Jun 8;15:100235. doi: 10.1016/j.xnsj.2023.100235. eCollection 2023 Sep.
3
The PI3K-Akt-mTOR and Associated Signaling Pathways as Molecular Drivers of Immune-Mediated Inflammatory Skin Diseases: Update on Therapeutic Strategy Using Natural and Synthetic Compounds.
PI3K-Akt-mTOR 及相关信号通路作为免疫介导性炎症性皮肤病的分子驱动因素：天然和合成化合物治疗策略的最新进展。
Cells. 2023 Jun 20;12(12):1671. doi: 10.3390/cells12121671.
4
Spinal cord injury: molecular mechanisms and therapeutic interventions.脊髓损伤：分子机制与治疗干预。
Signal Transduct Target Ther. 2023 Jun 26;8(1):245. doi: 10.1038/s41392-023-01477-6.
5
Boosting corticospinal system synaptic plasticity to recover motor functions.增强皮质脊髓系统的突触可塑性以恢复运动功能。
Neural Regen Res. 2023 Oct;18(10):2182-2183. doi: 10.4103/1673-5374.369103.
6
Mesenchymal stem cells in the treatment of spinal cord injury: Mechanisms, current advances and future challenges.间质干细胞治疗脊髓损伤：机制、当前进展和未来挑战。
Front Immunol. 2023 Feb 24;14:1141601. doi: 10.3389/fimmu.2023.1141601. eCollection 2023.
7
Synaptogenic gene therapy with FGF22 improves circuit plasticity and functional recovery following spinal cord injury.成突触基因治疗联合 FGF22 可改善脊髓损伤后的回路可塑性和功能恢复。
EMBO Mol Med. 2023 Feb 8;15(2):e16111. doi: 10.15252/emmm.202216111. Epub 2023 Jan 5.
8
The role of PI3K/Akt signalling pathway in spinal cord injury.PI3K/Akt 信号通路在脊髓损伤中的作用。
Biomed Pharmacother. 2022 Dec;156:113881. doi: 10.1016/j.biopha.2022.113881. Epub 2022 Oct 19.
9
The Neuroprotective Effects of Exosomes Derived from TSG101-Overexpressing Human Neural Stem Cells in a Stroke Model.人神经干细胞来源的 TSG101 过表达外泌体对脑卒中模型的神经保护作用。
Int J Mol Sci. 2022 Aug 23;23(17):9532. doi: 10.3390/ijms23179532.
10
Clinical translation of stem cell therapy for spinal cord injury still premature: results from a single-arm meta-analysis based on 62 clinical trials.临床转化中的干细胞治疗脊髓损伤仍不成熟：基于 62 项临床试验的单臂荟萃分析结果。
BMC Med. 2022 Sep 5;20(1):284. doi: 10.1186/s12916-022-02482-2.