Department of Pediatrics, School of Medicine, University of Missouri-Columbia, Columbia, MO 65211, USA.
Department of Health Science and Community, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.
Int J Mol Sci. 2024 Aug 11;25(16):8753. doi: 10.3390/ijms25168753.
Th1 and Th2 cytokines determine the outcome of infection and immune protection depends mainly on memory T cells induced during vaccination. This largely hinges on the nature and type of memory T cells produced. In this study, transgenic strains expressing membrane-associated ovalbumin (mOVA) and soluble ovalbumin (sOVA) were used as a model to study whether fully differentiated Th1/Th2 and Th17 cells can recall immune memory and tolerate pathogen manipulation. Naïve OT-II T cells were polarised in vitro into Th1/Th2 cells, and these cells were transferred adoptively into recipient mice. Following the transferral of the memory cells, the recipient mice were challenged with OVA transgenic and a wild-type parasite was used a control. The in vitro-polarised T helper cells continued to produce the same cytokine signatures after being challenged by both forms of OVA-expressing parasites in vivo. This suggests that antigen-experienced cells remain the same or unaltered in the face of OVA-transgenic Such ability of these antigen-experienced cells to remain resilient to manipulation by the parasite signifies that vaccines might be able to produce immune memory responses and defend against parasitic immune manipulation in order to protect the host from infection.
Th1 和 Th2 细胞因子决定感染的结果，而免疫保护主要依赖于疫苗接种过程中诱导的记忆 T 细胞。这在很大程度上取决于所产生的记忆 T 细胞的性质和类型。在这项研究中，表达膜结合卵清蛋白（mOVA）和可溶性卵清蛋白（sOVA）的转基因株系被用作模型，以研究完全分化的 Th1/Th2 和 Th17 细胞是否可以回忆免疫记忆并耐受病原体的操纵。幼稚的 OT-II T 细胞在体外被极化为 Th1/Th2 细胞，这些细胞被过继转移到受体小鼠中。在转移记忆细胞后，受体小鼠受到 OVA 转基因的挑战，野生型寄生虫被用作对照。在体内受到表达 OVA 的两种寄生虫形式的挑战后，体外极化的辅助性 T 细胞继续产生相同的细胞因子特征。这表明抗原经验细胞在面对 OVA 转基因时保持相同或不变。这些抗原经验细胞能够抵抗寄生虫的操纵的能力表明，疫苗可能能够产生免疫记忆反应，并抵御寄生虫的免疫操纵，以保护宿主免受感染。
