Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 1000 Sofia, Bulgaria.
Zoological Institute, Russian Academy of Sciences, 199034 St. Petersburg, Russia.
Genes (Basel). 2024 Aug 5;15(8):1026. doi: 10.3390/genes15081026.
Fluorescence in situ hybridization (FISH) with two different probes, the canonical insect telomeric sequence (TTAGG) and the sequence (TTAGGGATGG), was performed on meiotic chromosomes of two members of the true bug family Cimicidae (Cimicomorpha), the common bed bug Linnaeus, 1758 and the tropical bed bug . (Fabricius, 1803), whose telomeric motifs were not known. In both species, there were no hybridization signals with the first probe, but strong signals at chromosomal ends were observed with the second probe, indicating the presence of a telomeric motif (TTAGGGATGG). This study represents the first FISH confirmation of the presence of a non-canonical telomeric motif not only for the infraorder Cimicomorpha but also for the suborder Heteroptera (Hemiptera) as a whole. The present finding is of key significance for unraveling the evolutionary shifts in the telomeric sequences in this suborder.
使用两种不同的探针（经典昆虫端粒序列 TTAGG 和 TTAGGGATGG 序列）对两个盲蝽科（半翅目）成员的减数分裂染色体进行荧光原位杂交（FISH），这两个成员是常见的床虱 Linnaeus，1758 和热带床虱 （Fabricius，1803），它们的端粒序列尚不清楚。在这两个物种中，第一个探针都没有杂交信号，但第二个探针在染色体末端观察到强烈的信号，表明存在一个端粒序列（TTAGGGATGG）。这项研究不仅首次通过 FISH 证实了在亚目盲蝽下目（半翅目）中存在非经典的端粒序列，而且还证实了整个亚目（半翅目）的存在。这一发现对于揭示该亚目端粒序列的进化转变具有关键意义。
