Cunha Luciano B, Lepore Eloah D, Medeiros Camila Cristina B, Sorrechia Rodrigo, Pietro Rosemeire C L R, Corrêa Marcos A
School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14800-903, Brazil.
Life (Basel). 2024 Aug 17;14(8):1022. doi: 10.3390/life14081022.
Gentisic acid (2,5-dihydroxybenzoic acid) is primarily found naturally in plants and has demonstrated a significant range of biological activities; however, its efficacy and safety as a topical application ingredient are not yet well established. Thus, the compound's potential antioxidant and antimicrobial properties were evaluated for efficacy, while the cytotoxicity was evaluated for safety. The antioxidant activity, measured by the DPPH kinetic method, showed an Efficiency Concentration (EC) of 0.09 with an antioxidant reducing power (ARP) of 11.1. The minimum inhibitory concentration (MIC) against was 4.15 mg/mL, was 4.00 mg/mL, was 3.00 mg/mL, and was 3.60 mg/mL, and the MIC for has remained unpublished until now. The substance showed low cytotoxicity by the neutral red uptake (NRU) methodology against HaCat, HDFa, and HepG2 cells at concentrations of up to 10.0, 7.3, and 4.0 mM, respectively, also representing unpublished data. This evidence demonstrates gentisic acid as a promising active substance for skin topical application in the cosmetic or pharmaceutical industry.
龙胆酸（2,5 - 二羟基苯甲酸）主要天然存在于植物中，并已显示出广泛的生物活性；然而，其作为局部应用成分的功效和安全性尚未得到充分证实。因此，对该化合物的潜在抗氧化和抗菌特性进行了功效评估，同时对细胞毒性进行了安全性评估。通过DPPH动力学方法测量的抗氧化活性显示，其有效浓度（EC）为0.09，抗氧化还原能力（ARP）为11.1。对[具体菌种1]的最低抑菌浓度（MIC）为4.15 mg/mL，对[具体菌种2]为4.00 mg/mL，对[具体菌种3]为3.00 mg/mL，对[具体菌种4]为3.60 mg/mL，而对[具体菌种5]的MIC至今尚未发表。通过中性红摄取（NRU）方法，该物质在浓度分别高达10.0 mM、7.3 mM和4.0 mM时，对HaCat、HDFa和HepG2细胞显示出低细胞毒性，这些数据同样尚未发表。这一证据表明龙胆酸是化妆品或制药行业中用于皮肤局部应用的一种有前景的活性物质。
