Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China.
Hangzhou Island Xingqing Biotechnology Co., Ltd., Hangzhou 310023, China.
Molecules. 2024 Aug 10;29(16):3800. doi: 10.3390/molecules29163800.
Milk, on account of its abundant protein content, is recognized as a vital source of bioactive substances. In this study, the bioactive ingredients in milk were obtained by a combination of protease hydrolysis and fermentation with . The compositions of protease hydrolysate (PM) and fermentation supernatant (FM) were determined, and their anti-oxidant and anti-bacterial activities were evaluated. Using LC-MS/MS, the molecular weights and sequences of the peptides were characterized, among which a total of 25 bioactive peptides were identified. The DPPH radical scavenging results demonstrated that FM exhibited an enhanced anti-oxidant capacity compared to PM. The bacterial survival rate results revealed that FM had a remarkable anti-bacterial ability compared to PM. Additionally, the anti-bacterial component and potential anti-bacterial mechanisms were determined. The results of cytoplasmic membrane depolarization, cell membrane permeability, and morphological observation indicated that FM could interact with bacterial membranes to achieve its anti-bacterial effect. These findings suggested that FM, as a bioactive substance of natural origin, holds potential applications in the functional food, pharmaceutical, and cosmetic industries.
牛奶因其丰富的蛋白质含量而被认为是生物活性物质的重要来源。本研究采用蛋白酶水解和发酵相结合的方法从牛奶中获得生物活性成分。测定了蛋白酶水解产物（PM）和发酵上清液（FM）的组成，并评价了它们的抗氧化和抗菌活性。采用 LC-MS/MS 对肽的分子量和序列进行了表征，共鉴定出 25 种生物活性肽。DPPH 自由基清除结果表明，FM 的抗氧化能力比 PM 增强。细菌存活率结果表明，FM 对细菌的抑制作用明显优于 PM。此外，还确定了抗菌成分和潜在的抗菌机制。细胞质膜去极化、细胞膜通透性和形态观察的结果表明，FM 可以与细菌膜相互作用，从而达到其抗菌效果。这些发现表明，FM 作为一种天然来源的生物活性物质，在功能性食品、制药和化妆品行业具有潜在的应用前景。
