Salinas-Virgen Leilani Itzel, de la Torre-Hernández María Eugenia, Aguirre-Garrido José Félix, Martínez-Abarca Francisco, Ramírez-Saad Hugo César
Doctorado en Ciencias Agropecuarias, Universidad Autónoma Metropolitana-Xochimilco, Mexico City 04960, Mexico.
CONAHCYT-Universidad Autónoma Metropolitana-Xochimilco, Mexico City 04960, Mexico.
Microorganisms. 2024 Jul 24;12(8):1512. doi: 10.3390/microorganisms12081512.
Plant growth-promoting rhizobacteria (PGPR) are a group of bacteria that associate with the rhizosphere of plants; one of the most abundant bacterial genera in this ecological niche is , which is constantly expanding due to the emergence of new species such as , whose discovery in 2019 has led to the characterization of several strains from different environments but taxonomically related. The objective of this work was to phenotypically and molecularly characterize strain EMP42, isolated from the rhizosphere of . The strain EMP42 is able to use different substrates and reduce oxidative stress in plants. It is capable of improving growth parameters such as the number of inflorescences and the height of the aerial body of , as well as the germination and seedling survival of the cacti and . The genetic structure of EMP42 consists of a closed chromosome of 6.14 Mbp, and 61.1% GC content. It has 5572 genes, including those associated with PGPR activities, such as the ABCDE, SAP, ABPRU and ABC genes, among others, and three ncRNA loci, nine regulatory regions, five complete rRNA operons and three CRISPR-Cas loci, showing phylogenomic similarities with the reference strain B21-026. Therefore, this study contributes to the understanding of genomic diversity within and, particularly, highlights the potential application of strain EMP42 as a PGPR.
植物促生根际细菌（PGPR）是一类与植物根际相关的细菌；在这一生态位中最丰富的细菌属之一是[具体属名未给出]，由于新物种的出现，如[具体新物种名未给出]，该属不断扩大，其于2019年被发现，促使人们对来自不同环境但分类学上相关的几个菌株进行了表征。这项工作的目的是对从[具体植物名未给出]根际分离出的[具体菌株名未给出]EMP42菌株进行表型和分子特征分析。EMP42菌株能够利用不同底物并减轻植物中的氧化应激。它能够改善诸如[具体植物名未给出]的花序数量和地上部分高度等生长参数，以及仙人掌[具体仙人掌名未给出]和[具体仙人掌名未给出]的发芽和幼苗存活情况。EMP42的遗传结构由一条6.14 Mbp的闭合染色体组成，GC含量为61.1%。它有5572个基因，包括与PGPR活性相关的基因，如ABCDE、SAP、ABPRU和ABC基因等，以及三个非编码RNA基因座、九个调控区域、五个完整的rRNA操纵子和三个CRISPR-Cas基因座，与参考菌株[具体参考菌株名未给出]B21-026显示出系统发育基因组相似性。因此，本研究有助于了解[具体属名未给出]内的基因组多样性，特别是突出了EMP42菌株作为PGPR的潜在应用。
