Shi Nan, He Teng, Qin Huifang, Wang Ziye, You Shenghao, Wang Entao, Hu Guoli, Wang Fang, Yu Miao, Liu Xiaoyun, Liu Zhenyu
Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Engineering Research Center of Microbial Breeding and Conservation, Hebei Province, Institute of Life Science and Green Development, Hebei University, Baoding 071002, China.
Affiliation Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politecnico Nacional, Mexico City 11340, Mexico.
Microorganisms. 2024 Jul 30;12(8):1558. doi: 10.3390/microorganisms12081558.
Four pigment-producing rhizobial strains nodulating (Retz.) Poir. formed a unique group in genus in the phylogeny of a 16S rRNA gene and five housekeeping genes (, , , , and ) in a genome analysis, phenotypic characteristics analysis, and chemotaxonomic analysis. These four strains shared as high as 99.3% similarity with LmiM8 in the 16S rRNA gene sequence and, in an MLSA, were subdivided into two clusters, ANI (genome average nucleotide) and dDDH (digital DNA-DNA hybridization) which shared sequence similarities lower than the species thresholds with each other and with the reference strains for related species. The polar lipids elucidated that phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and cardiolipin were the main components for strain SWF67558 and for strain HBU65207, with the exception of PC. SWF67558 and HBU65207 strains had similar predominant cellular fatty acids, including C16:0, C18:0, summed feature 2, and summed feature8, but with different contents. In addition, all the four novel strains produced pink-pigment, and the main coloring material extract from strain SWF67558 was identified as zeaxanthin, which presented antioxidant ability and reduction power. With all the phylogenetic and phenotypic divergency, we proposed these pink-pigmented symbiotic bacteria as two novel species, named sp. nov. and sp. nov., with SWF67558 (=KCTC82331=GDMCC1.2024) and HBU65207 (=KCTC92125=GDMCC1.2023) as the type strains, respectively.
在一项基因组分析、表型特征分析和化学分类分析中，四株能使（Retz.）Poir.结瘤的产色素根瘤菌菌株在基于16S rRNA基因和五个管家基因（、、、和）的系统发育中，在属内形成了一个独特的类群。这四株菌株在16S rRNA基因序列上与LmiM8的相似度高达99.3%，并且在多位点序列分析（MLSA）中，被细分为两个聚类，即平均核苷酸一致性（ANI）和数字DNA-DNA杂交（dDDH），它们彼此之间以及与相关物种的参考菌株的序列相似性均低于物种阈值。极性脂质分析表明，磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）、磷脂酰甘油（PG）和心磷脂是菌株SWF67558的主要成分，而对于菌株HBU65207，除PC外也是如此。SWF67558和HBU65207菌株具有相似的主要细胞脂肪酸，包括C16:0、C18:0、总和特征2和总和特征8，但含量不同。此外，所有这四株新菌株均产生粉红色色素，并且从菌株SWF67558中提取的主要色素被鉴定为玉米黄质，其具有抗氧化能力和还原能力。鉴于所有的系统发育和表型差异，我们将这些粉红色色素共生细菌提议为两个新物种，分别命名为sp. nov.和sp. nov.，其中SWF67558（=KCTC82331=GDMCC1.2024）和HBU65207（=KCTC92125=GDMCC1.2023）分别作为模式菌株。
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