Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 139-774, Korea.
Division of Applied Food System, Major in Food Science & Technology, Seoul Women's University, Seoul, 01797, Korea.
Arch Microbiol. 2022 Jul 27;204(8):525. doi: 10.1007/s00203-022-03109-z.
Two novel Gram-stain-negative, aerobic, rod shaped bacterial strains BT290 and BT689 were isolated from soil collected in South Korea. Colony morphologies of both strains were circular and convex while the colors of BT290 and BT689 were light-pink and white, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that BT290 and BT689 belong to a distinct lineage within the genus Microvirga (family Methylobacteriaceae, order Rhizobiales, class Alphaproteobacteria, phylum Proteobacteria, kingdom Bacteria). The 16S rR NA gene sequence similarity between two strains was 97.9%. Both strains had the similar quinone system, with ubiquinone 10 (Q-10) as the major respiratory quinone. The major polar lipids of strains BT290 and BT689 were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), phosphatidylcholine (PC), and phosphatidylglycerol (PG). The major cellular fatty acids of strain BT290 were C ω7c (58.2%) and C (17.7%), while those of strain BT689 were C ω7c (61.8%) and C (10.8%). On the bases of polyphasic analysis (phylogenetic, chemotaxonomic, and biochemical), strains BT290 and BT689 can be suggested as novel bacterial species within the genus Microvirga and the proposed names are Microvirga terrestris and Microvirga arvi, respectively. The type strain of Microvirga terrestris is BT290 (= KCTC 72367 = NBRC 114844) and the type strain of Microvirga arvi is BT689 (= KACC 22016 = NBRC 114858), respectively.
两株新型革兰氏阴性、需氧、杆状细菌 BT290 和 BT689 是从韩国采集的土壤中分离得到的。两株菌的菌落形态均为圆形凸起，BT290 的颜色为浅粉色，BT689 的颜色为白色。基于 16S rRNA 基因序列的系统发育分析表明，BT290 和 BT689 属于微杆菌属（甲基杆菌科，根瘤菌目，α-变形菌纲，变形菌门，细菌域）的一个独特谱系。两株菌的 16S rRNA 基因序列相似性为 97.9%。两株菌的醌类系统相似，均以泛醌 10（Q-10）作为主要呼吸醌。菌株 BT290 和 BT689 的主要极性脂类为磷脂酰乙醇胺（PE）、双磷脂酰甘油（DPG）、磷脂酰胆碱（PC）和磷脂酰甘油（PG）。BT290 菌株的主要细胞脂肪酸为 C ω7c（58.2%）和 C 17:7（17.7%），BT689 菌株的主要细胞脂肪酸为 C ω7c（61.8%）和 C 10:8（10.8%）。基于多相分析（系统发育、化学分类和生化），BT290 和 BT689 两株菌可被认为是微杆菌属中的新型细菌种，分别命名为土微杆菌（Microvirga terrestris）和阿维微杆菌（Microvirga arvi）。土微杆菌的模式株为 BT290（=KCTC 72367=NBRC 114844），阿维微杆菌的模式株为 BT689（=KACC 22016=NBRC 114858）。
