Chen Ming, Zhai Lin, Schønning Kristian, Alpízar-Alpízar Warner, Larum Ole, Andersen Leif Percival, Holck Susanne, Friis-Møller Alice
Department of Clinical Microbiology, University Hospital of Southern Denmark, 6200 Aabenraa, Denmark.
Department of Clinical Microbiology, Copenhagen University Hospital, Rigshospitalet, 2100 Copenhagen, Denmark.
Microorganisms. 2024 Aug 6;12(8):1602. doi: 10.3390/microorganisms12081602.
-associated disease (CDAD) is a major healthcare-associated infection. New treatment options for CDAD are needed. A traditional Chinese medicinal formula, Huo Xiang Zheng Qi (HXZQ), was chosen to test against CDAD in a mouse model.
C57BL/6 mice were challenged with (ATCC 43255) orally; then received saline; vancomycin 25 mg/kg; or HXZQ in two different concentrations twice daily for 5 days. The animals' body weight; clinical signs; and survival rates were registered daily. Fecal pellets from each animal were taken for PCR analysis as a control of infection.
50% of the mice receiving saline died; 85.7% of the mice receiving vancomycin survived; 75% of the mice receiving HXZQ survived; and 87.5% of the mice receiving a 1:1 saline dilution of HXZQ survived. The HXZQ-treated groups were PCR positive with loads less than that of the untreated mice. The weight loss in the vancomycin plus HXZQ 1:1 treated group; the vancomycin-treated group; and the untreated group were 3.08%, 4.06%, and 9.62%, respectively.
our results showed that HXZQ can protect mice from CDAD-related death as effectively as vancomycin and the combination of vancomycin and HXZQ may give even better protection.
艰难梭菌相关性疾病（CDAD）是一种主要的医疗保健相关感染。需要针对CDAD的新治疗选择。选择了一种传统中药配方藿香正气（HXZQ）在小鼠模型中对CDAD进行测试。
将C57BL/6小鼠经口用艰难梭菌（ATCC 43255）攻击；然后分别给予生理盐水、25mg/kg万古霉素或两种不同浓度的HXZQ，每日两次，共5天。每天记录动物的体重、临床症状和存活率。采集每只动物的粪便颗粒进行PCR分析，作为感染的对照。
接受生理盐水的小鼠中有50%死亡；接受万古霉素的小鼠中有85.7%存活；接受HXZQ的小鼠中有75%存活；接受1:1生理盐水稀释的HXZQ的小鼠中有87.5%存活。经HXZQ治疗的组PCR呈阳性，载量低于未治疗的小鼠。万古霉素加1:1 HXZQ治疗组、万古霉素治疗组和未治疗组的体重减轻分别为3.08%、4.06%和9.62%。
我们的结果表明，HXZQ可以像万古霉素一样有效地保护小鼠免于CDAD相关死亡，万古霉素和HXZQ联合使用可能提供更好的保护。
