Zhang Huaiyu, Li Yunhui, Li Wei, Li Zhaocai, Zhou Jizhang, Tong Dewen
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, China.
Microorganisms. 2024 Aug 13;12(8):1656. doi: 10.3390/microorganisms12081656.
() is an important zoonotic pathogen that seriously endangers the development of animal husbandry. Vaccination is the most effective approach to preventing infection. We previously reported a recombinant ghost (rECG)-based vaccine that demonstrated outstanding protective efficacy. In this study, we further attempted to fuse the cholera toxin B subunit (CTB), a widely studied potent mucosal immune adjuvant, with macrophage infectivity potentiator (MIP), a candidate antigen of , on the surface of the rECG and explore its protective effect against infection. The MIP fusion protein was highly expressed in the rECGs, and the CTB-modified rECGs significantly induced the activation of mouse bone marrow-derived dendritic cells in vitro. Intranasal immunization with rECGs induced a Th1-biased cellular immune response. Compared to the rECGs without CTB, the CTB-modified rECGs induced higher concentrations of IgA in the serum and vaginal wash solution. Moreover, in a mouse infection model, the CTB-modified rECGs significantly improved the clearance efficiency of and reduced the pathological damage to the uterus. This study demonstrates that incorporating CTB into rECGs significantly enhances the immunogenic potential of the rECG vaccine and can significantly enhance its protective efficacy against a challenge.
（）是一种严重危害畜牧业发展的重要人畜共患病原体。接种疫苗是预防感染的最有效方法。我们之前报道了一种基于重组菌蜕（rECG）的疫苗，其显示出卓越的保护效力。在本研究中，我们进一步尝试将广泛研究的强效黏膜免疫佐剂霍乱毒素B亚基（CTB）与（）的候选抗原巨噬细胞感染增强因子（MIP）融合在rECG表面，并探究其对（）感染的保护作用。MIP融合蛋白在rECG中高表达，且CTB修饰的rECG在体外显著诱导小鼠骨髓来源树突状细胞的活化。用rECG进行鼻内免疫诱导了以Th1为主的细胞免疫反应。与未修饰CTB的rECG相比，CTB修饰的rECG在血清和阴道冲洗液中诱导产生更高浓度的IgA。此外，在小鼠感染模型中，CTB修饰的rECG显著提高了（）的清除效率，并减轻了对子宫的病理损伤。本研究表明，将CTB整合到rECG中可显著增强rECG疫苗的免疫原性潜力，并能显著增强其对（）攻击的保护效力。
