Regulatory Peptides-Energy Metabolism and Motivated Behavior Team, Neuroendocrine, Endocrine and Germinal Differentiation and Communication Laboratory, Inserm UMR1239, University of Rouen Normandie, 76000 Rouen, France.
Nutrients. 2024 Aug 13;16(16):2677. doi: 10.3390/nu16162677.
Before the absorption in the intestine, glucose encounters gut bacteria, which may serve as a barrier against hyperglycemia by metabolizing glucose. In the present study, we compared the capacity of enterobacterial strains to lower glucose levels in an in vitro model of nutrient-induced bacterial growth. Two probiotic strains, () and () , as well as , were studied. To mimic bacterial growth in the gut, a planktonic culture was supplemented twice daily by the Luria Bertani milieu with or without 0.5% glucose. Repeated nutrient provision resulted in the incremental growth of bacteria. However, in the presence of glucose, the maximal growth of both strains of but not of was inhibited. When glucose was added to the culture medium, a continuous decrease in its concentration was observed during each feeding phase. At its highest density, displayed more efficient glucose consumption accompanied by a more pronounced downregulation of glucose transporters' expression than . Thus, the study reveals that the probiotic strain is more resilient to maintain its growth than in the presence of 0.5% glucose accompanied by more efficient glucose consumption. This experimental approach offers a new strategy for the identification of probiotics with increased glucose metabolizing capacities potentially useful for the prevention and co-treatment of type 2 diabetes.
在肠道吸收之前，葡萄糖会遇到肠道细菌，这些细菌可能通过代谢葡萄糖来作为对抗高血糖的屏障。在本研究中，我们比较了肠杆菌菌株在营养诱导细菌生长的体外模型中降低葡萄糖水平的能力。研究了两种益生菌菌株（）和（）以及。为了模拟肠道中的细菌生长，浮游培养物每天两次用 Luria Bertani 培养基补充，有或没有 0.5%葡萄糖。反复提供营养物质导致细菌的递增生长。然而，在葡萄糖存在的情况下，两种 菌株的最大生长受到抑制，但 的最大生长不受影响。当葡萄糖被添加到培养基中时，在每次喂养阶段都观察到其浓度持续下降。在最高密度时，显示出比 更有效的葡萄糖消耗，同时葡萄糖转运蛋白的表达下调更为明显。因此，该研究表明，与 相比，益生菌菌株 在存在 0.5%葡萄糖的情况下更能抵抗生长，同时更有效地消耗葡萄糖。这种实验方法为鉴定具有增加的葡萄糖代谢能力的益生菌提供了一种新策略，这可能对 2 型糖尿病的预防和联合治疗有用。
