Barbosa Amanda de Oliveira, Gava Danielle, Tochetto Caroline, Ribeiro Leonardo Clasen, Bastos Ana Paula Almeida, Morés Marcos Antônio Zanella, Schaefer Rejane, de Lima Marcelo
Laboratório de Virologia e Imunologia, Universidade Federal de Pelotas, Capão do Leão 96160-000, RS, Brazil.
Embrapa Suínos e Aves, BR 153, Km 110, Distrito de Tamanduá, Concordia 89715-899, SC, Brazil.
Vaccines (Basel). 2024 Jul 26;12(8):845. doi: 10.3390/vaccines12080845.
(SVA) is a picornavirus that is endemic in swine, causing a vesicular disease clinically indistinguishable from other vesicular diseases, like foot-and-mouth disease. The widespread viral circulation, constant evolution, and economic losses caused to the swine industry emphasize the need for measures to control the agent. In this study, we evaluated the immunogenicity of a whole-virus-inactivated vaccine using a representative contemporary Brazilian SVA strain in Balb/ByJ mice. The animals were vaccinated with two doses by an intramuscular route. The humoral response induced by the vaccination was evaluated by an in-house ELISA assay for IgG detection. The cellular response was assessed by flow cytometry after in vitro SVA stimulation in splenocyte cultures from vaccinated and non-vaccinated groups. Protection against SVA was assessed in the experimental groups following an oral challenge with the homologous virus. The vaccination induced high levels of IgG antibodies and the proliferation of CD45R/B220sIgM, CD3eCD69, and CD3eCD4CD44CD62L cells. These results indicate the immunogenicity and safety of the vaccine formulation in a murine model and the induction of humoral and cellular response against SVA.
猪水疱病病毒（SVA）是一种在猪群中流行的小RNA病毒，可引发一种临床上与其他水疱病难以区分的水疱病，如口蹄疫。病毒的广泛传播、不断进化以及给养猪业造成的经济损失凸显了采取措施控制该病原体的必要性。在本研究中，我们使用一株具有代表性的当代巴西SVA毒株，在Balb/ByJ小鼠中评估了全病毒灭活疫苗的免疫原性。通过肌肉注射途径给动物接种两剂疫苗。通过一种用于检测IgG的内部ELISA试验评估疫苗接种诱导的体液反应。在对接种组和未接种组的脾细胞培养物进行体外SVA刺激后，通过流式细胞术评估细胞反应。在用同源病毒进行口服攻毒后，在实验组中评估对SVA的保护作用。疫苗接种诱导了高水平的IgG抗体以及CD45R/B220sIgM、CD3eCD69和CD3eCD4CD44CD62L细胞的增殖。这些结果表明该疫苗制剂在小鼠模型中的免疫原性和安全性，以及诱导了针对SVA的体液和细胞反应。
