Pickering A J, Fink G
J Endocrinol. 1979 Oct;83(1):53-9.
The size of the 'readily releasable pool' of luteinizing hormone at various times of the oestrous cycle has been determined by injecting a supramaximal dose of luteinizing hormone releasing factor (LH-RF) i.v. into rats anaesthetized with sodium pentobarbitone. In an attempt to block replenishment of the 'pool' during release, cycloheximide was administered 30 min before LH-RF. A 20-fold increase in pool size occurred between the morning of dioestrus and the evening of pro-oestrus in the absence of any significant change in total pituitary content of LH. This suggests that increased responsiveness may be brought about by a change in the receptor-release apparatus and/or a transfer of LH from a 'storage pool' which leads to an apparent increase in the proportion of LH available for release.
通过向用戊巴比妥钠麻醉的大鼠静脉注射超最大剂量的促黄体生成素释放因子（LH-RF），测定了发情周期不同时间促黄体生成素“易释放池”的大小。为了在释放过程中阻断“池”的补充，在注射LH-RF前30分钟给予环己酰亚胺。在间情期早晨到发情前期晚上之间，“池”大小增加了20倍，而垂体中LH的总含量没有任何显著变化。这表明，反应性增加可能是由受体-释放装置的变化和/或LH从“储存池”的转移引起的，这导致可用于释放的LH比例明显增加。
