Xie Z M, Mao Q F, Xu M H, Gu P D, Xie Z J
Shanghai Institute of Planned Parenthood Research, WHO Collaborating Center for Research in Human Reproduction.
Sheng Li Xue Bao. 1997 Dec;49(6):649-56.
The intrinsic nature of rthymic release of luteinizing hormone (LH) of isolated human and rat anterior pituitary gland reported independently by Macro Gambacciani and Xie in 1987 can be more directly demonstrated by a computer programme of Time Series-HSY Hidden Periodic Analytic Approach for continuous monitoring the LH output of the perfusate from a perfusion system with in vitro anterior pituitary of SD female rat. The results are as follows: (1) Under various reproductive conditions the average frequency (min/cycle) and amplitude (ng/ml) of the intrinsic rhythm of LH release were quite different: In proestrous group the frequency and amplitude were the highest, being intermediate in the ovariectomized group and lowest in the lactation group. (2) The intrinsic rhythm of LH release could be changed by either peptide or steroid hormones. In proestrous group with 30 min of gonadotropin-releasing hormone (GnRH), stimulation would reduce both frequency and amplitude. In case of lactation, the frequency was unchanged, but amplitude lowered, while in the ovariectomized rat pituitary, the 30 min GnRH stimulation decreased the frequency of release only. The intrinsic rhythm of the LH release could also be influenced by steriod hormones (Ru486 and Anordrin). With 120 min before removal of the anterior pituitary gland the rats receiving i.m. injection of Ru486 (2 mg/kg bw) or Anordrin (2 mg/kg), the results showed that Ru486 decreased frequency, while Anordrin decreased only the frequency to a less extent, both without amplitude affected. (3) Verapamil and EGTA added to the perfusion system did not abolish but only decreased the rhythmic phenomenon by using proestrous pitutary. This suggests that participation of Ca2+ may take place in the intrinsic release of LH. The above results indicated that the intrinsic rhythm of LH release of isolated anterior pituitary gland is different from various reproductive hormonal conditions and capable of being modified by exogenous hormones. The physiological function of the intrinsic rhythm of LH release of anterior pituitary gland remains to be elucidated.
1987年，马尔科·甘巴奇亚尼和谢分别独立报道了分离出的人体和大鼠垂体前叶促黄体生成素（LH）的节律性释放的内在特性，通过一个时间序列-HSY隐周期分析方法的计算机程序可以更直接地证明这一点，该程序用于连续监测来自SD雌性大鼠体外垂体灌注系统的灌注液中LH的输出。结果如下：（1）在不同的生殖条件下，LH释放的内在节律的平均频率（分钟/周期）和幅度（纳克/毫升）有很大差异：在发情前期组，频率和幅度最高，在去卵巢组居中，在哺乳期组最低。（2）LH释放的内在节律可被肽类或甾体激素改变。在发情前期组给予促性腺激素释放激素（GnRH）刺激30分钟，频率和幅度均降低。在哺乳期，频率不变，但幅度降低，而在去卵巢大鼠垂体中，30分钟的GnRH刺激仅降低释放频率。LH释放的内在节律也可受甾体激素（米非司酮和双炔失碳酯）影响。在摘除垂体前120分钟，给大鼠肌肉注射米非司酮（2毫克/千克体重）或双炔失碳酯（2毫克/千克），结果显示米非司酮降低频率，而双炔失碳酯仅在较小程度上降低频率，两者均不影响幅度。（3）向灌注系统中加入维拉帕米和乙二醇双四乙酸（EGTA）并没有消除而是仅降低了发情前期垂体的节律现象。这表明Ca2+可能参与了LH的内在释放。上述结果表明，分离出的垂体前叶LH释放的内在节律因不同的生殖激素条件而异，并且能够被外源激素改变。垂体前叶LH释放内在节律的生理功能仍有待阐明。
