Jiang Dongbo, Zhang Junqi, Shen Wenyang, Sun Yubo, Wang Zhenjie, Wang Jiawei, Zhang Jinpeng, Zhang Guanwen, Zhang Gefei, Wang Yueyue, Cai Sirui, Zhang Jiaxing, Wang Yongkai, Liu Ruibo, Bai Tianyuan, Sun Yuanjie, Yang Shuya, Ma Zilu, Li Zhikui, Li Jijin, Ma Chenjin, Cheng Linfeng, Sun Baozeng, Yang Kun
Department of Immunology, The Key Laboratory of Bio-Hazard Damage and Prevention Medicine, Basic Medicine School, Air-Force Medical University (the Fourth Military Medical University), Xi'an 710032, China.
Department of Microbiology, Basic Medicine School, Air-Force Medical University (the Fourth Military Medical University), Xi'an 710032, China.
Vaccines (Basel). 2024 Aug 19;12(8):928. doi: 10.3390/vaccines12080928.
Vaccines has long been the focus of antiviral immunotherapy research. Viral epitopes are thought to be useful biomarkers for immunotherapy (both antibody-based and cellular). In this study, we designed a novel vaccine molecule, the Hantaan virus (HTNV) glycoprotein (GP) tandem Th epitope molecule (named the Gnc molecule), in silico. Subsequently, computer analysis was used to conduct a comprehensive and in-depth study of the various properties of the molecule and its effects as a vaccine molecule in the body. The Gnc molecule was designed for DNA vaccines and optimized with a lysosomal-targeting membrane protein (LAMP) strategy. The effects of GP-derived Th epitopes and multiepitope vaccines were initially verified in animals. Our research has resulted in the design of two vaccines based on effective antiviral immune targets. The effectiveness of molecular therapies has also been preliminarily demonstrated in silico and in laboratory animals, which lays a foundation for the application of a vaccines strategy in the field of antivirals.
疫苗长期以来一直是抗病毒免疫疗法研究的重点。病毒表位被认为是免疫疗法（基于抗体和细胞的）有用的生物标志物。在本研究中，我们在计算机上设计了一种新型疫苗分子，即汉坦病毒（HTNV）糖蛋白（GP）串联Th表位分子（命名为Gnc分子）。随后，利用计算机分析对该分子的各种特性及其作为体内疫苗分子的作用进行了全面深入的研究。Gnc分子是为DNA疫苗设计的，并采用溶酶体靶向膜蛋白（LAMP）策略进行了优化。最初在动物身上验证了GP衍生的Th表位和多表位疫苗的效果。我们的研究设计了两种基于有效抗病毒免疫靶点的疫苗。分子疗法的有效性也已在计算机模拟和实验动物中得到初步证明，这为疫苗策略在抗病毒领域的应用奠定了基础。
