Cho Kyung-Hyun, Lee Yunki, Lee Sang Hyuk, Kim Ji-Eun, Bahuguna Ashutosh
Raydel Research Institute, Medical Innovation Complex, Daegu 41061, Republic of Korea.
Pharmaceuticals (Basel). 2024 Aug 22;17(8):1103. doi: 10.3390/ph17081103.
Policosanol is a blend of long-chain aliphatic alcohols (LCAAs) and is well-known for several health-beneficial activities; however, the functionality of policosanol varied substantially based on the composition of LCAAs. In this study, two distinct policosanols, Raydel (extracted from Cuban sugarcane wax) and BOC Sciences (extracted from Chinese sugarcane wax), were dietarily supplemented (0.1% /) for 12 weeks in hyperlipidemic zebrafish to examine their influence on the blood lipid profile and functionality of the liver, kidney, and reproductive organs. The results demonstrated a noteworthy impact of both policosanols on preventing high-cholesterol diet (HCD, 4% /)-induced dyslipidemia by decreasing total cholesterol (TC) and triglyceride (TG) levels in the plasma. However, compared to BOC Sciences, the Raydel policosanol exhibited a significantly ( < 0.05) higher efficacy in reducing HCD-induced TC and TG levels. A substantial effect was observed exclusively with the Raydel policosanol in mitigating HCD-impaired low-density-lipoprotein cholesterol (LDL-C) and high-density-lipoprotein cholesterol (HDL-C) levels. Hepatic histology and immunohistochemistry (IHC) analysis revealed the higher efficacy of Raydel policosanol over BOC Sciences policosanol to prevent HCD-provoked fatty liver changes, cellular senescence, oxidative stress, and interleukin (IL)-6 production. Consistently, a significantly higher effect of Raydel over BOC Sciences policosanol was observed on the protection of kidney, testis, and ovary morphology hampered by HCD consumption. In addition, Raydel policosanol exhibited a notably stronger effect (~2-fold, < 0.05) on the egg-laying ability of the zebrafish compared to policosanol from BOC Sciences. Furthermore, Raydel policosanol plays a crucial role in improving embryo viability and mitigating developmental defects caused by the intake of an HCD. Conclusively, Raydel policosanol displayed a substantially higher efficacy over BOC Sciences policosanol to revert HCD-induced dyslipidemia, the functionality of vital organs, and the reproductive health of zebrafish.
聚二十碳醇是一种长链脂肪醇（LCAAs）的混合物，因其多种有益健康的活性而闻名；然而，聚二十碳醇的功能因LCAAs的组成而有很大差异。在本研究中，将两种不同的聚二十碳醇，即Raydel（从古巴甘蔗蜡中提取）和BOC Sciences（从中国甘蔗蜡中提取），以0.1%（/）的剂量添加到高脂血症斑马鱼的饮食中，持续12周，以研究它们对血脂谱以及肝脏、肾脏和生殖器官功能的影响。结果表明，两种聚二十碳醇均对预防高胆固醇饮食（HCD，4%（/））诱导的血脂异常有显著影响，可降低血浆中的总胆固醇（TC）和甘油三酯（TG）水平。然而，与BOC Sciences相比，Raydel聚二十碳醇在降低HCD诱导的TC和TG水平方面表现出显著更高的功效（<0.05）。仅在Raydel聚二十碳醇中观察到对减轻HCD损害的低密度脂蛋白胆固醇（LDL-C）和高密度脂蛋白胆固醇（HDL-C）水平有显著效果。肝脏组织学和免疫组织化学（IHC）分析显示，Raydel聚二十碳醇在预防HCD引发的脂肪肝变化、细胞衰老、氧化应激和白细胞介素（IL）-6产生方面比BOC Sciences聚二十碳醇具有更高的功效。同样，在保护因食用HCD而受损的肾脏、睾丸和卵巢形态方面，Raydel聚二十碳醇比BOC Sciences聚二十碳醇的效果显著更高。此外，与BOC Sciences的聚二十碳醇相比，Raydel聚二十碳醇对斑马鱼的产卵能力表现出明显更强的效果（约2倍，<0.05）。此外，Raydel聚二十碳醇在提高胚胎活力和减轻因摄入HCD引起的发育缺陷方面起着关键作用。总之，Raydel聚二十碳醇在逆转HCD诱导的血脂异常、重要器官功能和斑马鱼生殖健康方面比BOC Sciences聚二十碳醇表现出显著更高的功效。
