Chen Yuying, Lu Huangjie, He Qingwei, Yang Jie, Lu Hong, Han Jiongming, Zhu Ying, Hu Ping
College of Pharmacy, Jinan University, Guangzhou 511436, China.
International School, Jinan University, Guangzhou 511436, China.
Pharmaceutics. 2024 Jul 31;16(8):1019. doi: 10.3390/pharmaceutics16081019.
Accurately measuring drug and its release kinetics in both in vitro and in vivo environments is crucial for enhancing therapeutic effectiveness while minimizing potential side effects. Nevertheless, the real-time visualization of drug release from microspheres to monitor potential overdoses remains a challenge. The primary objective of this investigation was to employ fluorescence imaging for the real-time monitoring of drug release from microspheres in vitro, thereby simplifying the laborious analysis associated with the detection of drug release. Two distinct varieties of microspheres were fabricated, each encapsulating different drugs within PLGA polymers. Cy5 was selected as the donor, and Cy7 was selected as the acceptor for visualization and quantification of the facilitated microsphere drug release through the application of the fluorescence resonance energy transfer (FRET) principle. The findings from the in vitro experiments indicate a correlation between the FRET fluorescence alterations and the drug release profiles of the microspheres.
在体外和体内环境中准确测量药物及其释放动力学对于提高治疗效果同时将潜在副作用降至最低至关重要。然而，实时可视化微球中的药物释放以监测潜在的药物过量仍然是一项挑战。本研究的主要目的是利用荧光成像对微球在体外的药物释放进行实时监测，从而简化与药物释放检测相关的繁琐分析。制备了两种不同的微球，每种微球在PLGA聚合物中包裹不同的药物。选择Cy5作为供体，选择Cy7作为受体，通过应用荧光共振能量转移（FRET）原理对微球药物的促进释放进行可视化和定量。体外实验结果表明FRET荧光变化与微球的药物释放曲线之间存在相关性。
