Karnopp Juliana Camila Fischer, Jorge Juliana, da Silva Jaqueline Rodrigues, Boldo Diego, Del Pino Santos Kristiane Fanti, Duarte Adriana Pereira, de Castro Gustavo Rocha, de Azevedo Ricardo Bentes, Prada Ariadna Lafourcade, Amado Jesús Rafael Rodríguez, Martines Marco Antonio Utrera
Postgraduate Program in Chemistry, Chemistry Institute, Federal University of Mato Grosso do Sul, Campo Grande 79079-900, MS, Brazil.
Postgraduate Program in Nanoscience and Nanotechnology, Biological Science Institute, University of Brasilia, Brasilia 70910-900, DF, Brazil.
Pharmaceutics. 2024 Aug 19;16(8):1086. doi: 10.3390/pharmaceutics16081086.
This study describes the synthesis and characterization of chlorambucil (CLB)-functionalized mesoporous silica nanoparticles (MSNs) for potential application in cancer therapy. The nanoparticles were designed with a diameter between 20 and 50 nm to optimize cellular uptake and avoid rapid clearance from the bloodstream. The synthesis method involved modifying a previously reported technique to reduce particle size. Successful functionalization with CLB was confirmed through various techniques, including Fourier transform infrared spectroscopy (FTIR) and elemental analysis. The cytotoxicity of the CLB-functionalized nanoparticles (MSN@NH-CLB) was evaluated against human lung adenocarcinoma cells (A549) and colon carcinoma cells (CT26WT). The results suggest significantly higher cytotoxicity of MSN@NH-CLB compared to unbound CLB, with improved selectivity towards cancer cells over normal cells. This suggests that MSN@NH-CLB holds promise as a drug delivery system for targeted cancer therapy.
本研究描述了用于癌症治疗潜在应用的苯丁酸氮芥（CLB）功能化介孔二氧化硅纳米颗粒（MSN）的合成与表征。设计的纳米颗粒直径在20至50纳米之间，以优化细胞摄取并避免从血液中快速清除。合成方法涉及修改先前报道的技术以减小颗粒尺寸。通过包括傅里叶变换红外光谱（FTIR）和元素分析在内的各种技术证实了CLB的成功功能化。评估了CLB功能化纳米颗粒（MSN@NH-CLB）对人肺腺癌细胞（A549）和结肠癌细胞（CT26WT）的细胞毒性。结果表明，与未结合的CLB相比，MSN@NH-CLB的细胞毒性显著更高，对癌细胞的选择性优于正常细胞。这表明MSN@NH-CLB有望作为一种靶向癌症治疗的药物递送系统。
