Spartali Christina, Psarra Anna-Maria G, Marras Sotirios I, Tsioptsias Costas, Georgantopoulos Achilleas, Kalousi Foteini D, Tsakalof Andreas, Tsivintzelis Ioannis
Department of Biochemistry and Biotechnology, University of Thessaly, 41500 Larissa, Greece.
Department of Chemical Engineering, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Polymers (Basel). 2024 Aug 19;16(16):2346. doi: 10.3390/polym16162346.
Silybin is a natural flavonolignan with potential anticancer, antioxidant, and hepatoprotective properties. In the present study, various loadings of silybin (1, 3, and 5 wt%) were encapsulated in poly-ε-caprolactone (PCL) fibers by electrospinning, in order to produce new pharmaceutical composites with improved bioactive and drug delivery properties. The morphological characteristics of the composite fibrous structures were evaluated by scanning electron microscopy (SEM), and the encapsulation efficiency and the release rate of silybin were quantified using a UV-Vis spectrophotometer. The analysis of the membranes' thermal behavior by differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) revealed the existence of interaction between PCL and silybin. An investigation of the cytocompatibility of the composite membranes revealed that normal cells displayed an unimpeded proliferation in the respective silybin concentrations; however, tumor cell growth demonstrated a dose-dependent inhibition. Furthermore, an effective antioxidant activity against hydrogen peroxide-induced oxidative stress in HEK-293 cells was observed for the prepared electrospun fibrous mats.
水飞蓟宾是一种天然黄酮木脂素，具有潜在的抗癌、抗氧化和保肝特性。在本研究中，通过静电纺丝将不同载量（1%、3%和5%重量）的水飞蓟宾包裹在聚ε-己内酯（PCL）纤维中，以制备具有改善的生物活性和药物递送特性的新型药物复合材料。通过扫描电子显微镜（SEM）评估复合纤维结构的形态特征，并使用紫外可见分光光度计对水飞蓟宾的包封效率和释放速率进行定量。通过差示扫描量热法（DSC）和热重分析（TGA）对膜的热行为进行分析，揭示了PCL与水飞蓟宾之间存在相互作用。对复合膜的细胞相容性进行研究发现，正常细胞在各自的水飞蓟宾浓度下增殖不受阻碍；然而，肿瘤细胞生长表现出剂量依赖性抑制。此外，观察到所制备的静电纺丝纤维垫对过氧化氢诱导的HEK-293细胞氧化应激具有有效的抗氧化活性。
