A First Approach for the In Vitro Cultivation, Storage, and DNA Barcoding of the Endangered Endemic Species .

is a rare and protected species in Kazakhstan, valued for its ecological role in soil stabilization and its ornamental properties. This study presents the first use of micropropagation and phylogenetic analysis for the endemic plant . Seedlings of proved to be more effective than internodes as primary explants for plant micropropagation of in vitro culture, with a multiplication coefficient of 28.5 from seedlings and 6.1 from internodes. On MSR I medium supplemented with 0.5 mg/L IBA and 0.05 mg/L IAA, a higher success rate of 67% was achieved for root formation of test tube-grown plants. Using mannitol as an osmotic agent at a concentration of 8 mg/L prolonged the storage time of under slow growth conditions when compared to CCC and abscisic acid. Phylogenetic relationships and species identification were analyzed using four DNA-barcoding markers, comparing with species from NCBI. All candidate barcoding markers showed sufficient levels of interspecific genetic variation among species. In addition, ITS region and gene sequences effectively distinguished from other species. These results provide fundamental information that will be valuable for future biotechnological and molecular studies.