Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou, 510642, China.
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.
Sci Rep. 2019 Jul 3;9(1):9634. doi: 10.1038/s41598-019-46050-w.
Magnolia sirindhorniae Noot. & Chalermglin is an endangered species with high ornamental and commercial value that needs to be urgently protected and judiciously commercialized. In this study, a protocol for efficient regeneration of this species is standardized. The lateral buds of the M. sirindhorniae plant were used as an explant. Half-strength Murashige and Skoog (MS) medium supplemented with 2.0 mg/L 6-benzyladenine (BA), 0.1 mg/L α-naphthaleneacetic acid (NAA), and 2.0 mg/L gibberellic acid (GA) was found to be the optimal medium for shoot induction. The maximum shoot multiplication rate (310%) was obtained on Douglas-fir cotyledon revised medium (DCR) fortified with 0.2 mg/L BA, 0.01 mg/L NAA, and additives. The half-strength DCR medium supplemented with 0.5 mg/L NAA and 0.5 mg/L indole-3-butyric acid (IBA) supported the maximum rate (85.0%) of in vitro root induction. After a simple acclimatization process, the survival rate of plantlets in a substrate mixture of sterile perlite and peat soil (1:3; v/v) was 90.2%. DNA markers were used for assessment of genetic uniformity, confirming the genetic uniformity and stability of regenerated plants of M. sirindhorniae. Thus, the described protocol can safely be applied for large scale propagation of this imperative plant.
含笑属的一种植物，学名为 Magnolia sirindhorniae Noot. & Chalermglin，是一种具有较高观赏和商业价值的濒危物种，需要紧急保护和明智地商业化。在本研究中，标准化了一种高效再生该物种的方案。以含笑属植物的侧芽为外植体。结果表明，半强度 Murashige 和 Skoog (MS) 培养基添加 2.0 mg/L 6-苄基腺嘌呤 (BA)、0.1 mg/Lα-萘乙酸 (NAA) 和 2.0 mg/L 赤霉素 (GA) 是诱导芽的最佳培养基。在添加 0.2 mg/L BA、0.01 mg/L NAA 和添加剂的道格拉斯冷杉子叶修订培养基 (DCR) 上，获得了最高的芽增殖率 (310%)。在半强度 DCR 培养基中添加 0.5 mg/L NAA 和 0.5 mg/L 吲哚丁酸 (IBA) 支持离体生根的最高诱导率 (85.0%)。在简单的驯化过程后，无菌珍珠岩和泥炭土 (1:3; v/v) 基质混合物中植株的成活率为 90.2%。使用 DNA 标记物评估遗传均匀性，确认了再生的 Magnolia sirindhorniae 植物的遗传均匀性和稳定性。因此，所描述的方案可以安全地应用于该迫切需要的植物的大规模繁殖。
